Objective

This study investigated the effect of silver diammine fluoride (SDF), nano-silver (AgNPs) and nano-silver/potassium fluoride (AgNPs/KF) on the morphology of dentin collagen and color staining on the dentin. Also, we investigated the anti-caries properties in terms of anti-bacterial effects and potentiality of dentin remineralization at collagen level.

Methods

Dentin specimens obtained from bovine tooth roots were finished with #2000 SiC and sonicated for 5 min. Specimens were divided into two main groups: sound and demineralized dentin. Each group was subdivided into five subgroups according to treatment solution: control (no treatment), SDF, nano-silver (AgNPs), AgNPs/KF and KF. Specimens were analyzed using transmission electron microscope (TEM), spectrophotometer, scanning electron microscope (SEM) and antibacterial test. Repeated Measures ANOVA was used for statistical analysis of color change while one-way ANOVA was used for CFU analysis.

Results

Regarding color change, SDF showed the highest values while the other groups exhibited insignificant changes. SDF showed alteration in the collagen fibrils while the other groups showed normal intact fibrils. Regarding antibacterial activity, SDF and AgNPs/KF showed the greatest antibacterial effect.

Significance

SDF altered the dentin collagen morphology and inferior ethetics, but still providing intrafibrillar remineralization. Nano-silver fluoride preparations (AgNPs/KF) can be an alternative material for SDF as it can preserve the dentin collagen, showing no color change overtime, and provide a high anti-caries property including intrafibrillar remineralization with a significant anti-bacterial effect.

中文翻译：

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含银剂对牙本质胶原超微结构形态的影响。

目的

这项研究调查了二胺氟化银（SDF），纳米银（AgNPs）和纳米银/氟化钾（AgNPs / KF）对牙本质胶原蛋白形态和牙本质染色的影响。此外，我们从抗菌效果和牙本质在胶原水平上再矿化的潜力方面研究了抗龋齿性能。

方法

从牛牙根获得的牙本质标本用＃2000 SiC抛光并超声处理5分钟。标本分为两大类：牙本质和脱矿质牙本质。根据治疗方案将每组分为五个亚组：对照（不治疗），SDF，纳米银（AgNP），AgNP / KF和KF。使用透射电子显微镜（TEM），分光光度计，扫描电子显微镜（SEM）和抗菌测试分析样品。重复测量ANOVA用于颜色变化的统计分析，而单向ANOVA用于CFU分析。

结果

关于颜色变化，SDF显示最高值，而其他组则显示不明显的变化。SDF显示胶原原纤维的改变，而其他组则显示正常的完整原纤维。关于抗菌活性，SDF和AgNPs / KF表现出最大的抗菌作用。

意义

SDF改变了牙本质胶原的形态和较差的美学，但仍提供了原纤维内再矿化作用。纳米氟化银制剂（AgNPs / KF）可以作为SDF的替代材料，因为它可以保存牙本质胶原蛋白，不随时间变化而显示颜色变化，并具有很高的防龋性能，包括纤维内再矿化和显着的抗菌作用。