Short-term treatment of mammalian oocytes with different stressors induces stress tolerance of embryos derived from these oocytes. The aims of this study were to evaluate effects on embryo development when there was treatment of oocyte complexes (COCs) used to derive the embryos with hydrogen peroxide (H₂O₂).The COCs were not incubated with H₂O₂: control (0 μM), or were incubated with 25, 50, 75, or 100 μM concentrations of H₂O₂ for 1 h prior to in vitro fertilization, and presumptive zygotes were cultured until day 7. Blastocysts at day 7 of development derived from H₂O₂-treated (25 μM treatment concentration) COCs were vitrified. Percentage of embryos undergoing cleavage was not affected by any treatment, while percentage of embryos developing to the blastocyst stage was less when there was treatment of COCs with 100 μM of H₂O₂. Embryo quality was less when COCs used to derive blastocysts were treated with 50, 75, or 100 μM concentrations of H₂O₂. There were lesser relative abundances of some mRNA transcripts of interest in blastocysts when there was treatment of COCs with H₂O₂. After vitrification, there were no differences in embryo re-expansion and hatching rates compared with fresh and vitrified blastocysts of the control group and those derived from COCs treated with 25 μM H₂O₂. In conclusion, treatment of COCs used to derive blastocysts with H₂O₂ does not induce stress tolerance in vitrified embryos of cattle; however, the viability of these blastocysts is similar to those of the control group.

用不同的应激源短期处理哺乳动物卵母细胞会诱导源自这些卵母细胞的胚胎的压力耐受性。这项研究的目的是评估使用过氧化氢（H ₂ O ₂）处理用于衍生胚胎的卵母细胞复合物（COC）时对胚胎发育的影响。未将COC与H ₂ O ₂：对照（ （0μM），或在体外受精前与25、50、75或100μM浓度的H ₂ O ₂孵育1 h ，然后将假定的受精卵培养至第7天。在发育的第7天，来自H的囊胚₂ O ₂将经处理的（25μM处理浓度）COC玻璃化。接受卵裂的胚胎的百分比不受任何处理的影响，而当用100μMH ₂ O ₂处理COC时，发育到胚泡期的胚胎的百分比则较小。当使用50、75或100μM浓度的H ₂ O ₂处理用于衍生胚泡的COC时，胚胎质量会降低。当用H ₂ O ₂处理COC时，胚泡中某些目的mRNA转录本的相对丰度相对较低。。玻璃化后，与对照组的新鲜和玻璃化胚泡以及源自用25μMH ₂ O ₂处理的COC的胚泡相比，胚胎的再扩增和孵化率没有差异。总之，用牛H ₂ O ₂处理用于衍生胚泡的COC不会在牛的玻璃化胚胎中诱导胁迫耐受性。但是，这些胚泡的生存能力与对照组相似。