Background

Unfavorable climatic changes have led to an increased threat of several biotic and abiotic stresses over the past few years. Looking at the massive damage caused by these stresses, we undertook a study to develop high yielding climate-resilient rice, using genes conferring resistance against blast (Pi9), bacterial leaf blight (BLB) (Xa4, xa5, xa13, Xa21), brown planthopper (BPH) (Bph3, Bph17), gall midge (GM) (Gm4, Gm8) and QTLs for drought tolerance (qDTY_1.1 and qDTY_3.1) through marker-assisted forward breeding (MAFB) approach.

Result

Seven introgression lines (ILs) possessing a combination of seven to ten genes/QTLs for different biotic and abiotic stresses have been developed using marker-assisted selection (MAS) breeding method in the background of Swarna with drought QTLs. These ILs were superior to the respective recurrent parent in agronomic performance and also possess preferred grain quality with intermediate to high amylose content (AC) (23–26%). Out of these, three ILs viz., IL1 (Pi9+ Xa4+ xa5+ Xa21+ Bph17+ Gm8+ qDTY_1.1+ qDTY_3.1), IL6 (Pi9+ Xa4+ xa5+ Xa21+ Bph3+ Bph17+ Gm4+ Gm8+ qDTY_1.1+ qDTY_3.1) and IL7 (Pi9+ Xa4+ xa5+ Bph3+ Gm4+ qDTY_1.1+ qDTY_3.1) had shown resistance\tolerance for multiple biotic and abiotic stresses both in the field and glasshouse conditions. Overall, the ILs were high yielding under various stresses and importantly they also performed well in non-stress conditions without any yield penalty.

Conclusion

The current study clearly illustrated the success of MAS in combining tolerance to multiple biotic and abiotic stresses while maintaining higher yield potential and preferred grain quality. Developed ILs with seven to ten genes in the current study showed superiority to recurrent parent Swarna+drought for multiple-biotic stresses (blast, BLB, BPH and GM) together with yield advantages of 1.0 t ha^− 1 under drought condition, without adverse effect on grain quality traits under non-stress.

中文翻译：

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标记辅助正向育种结合水稻的多种生物-非生物胁迫抗性/耐性。

背景

在过去的几年中，不利的气候变化导致多种生物和非生物胁迫的威胁增加。考虑到这些压力造成的巨大破坏，我们进行了一项研究，开发了高产耐气候性水稻，利用赋予抗稻瘟病（Pi9），细菌性叶枯病（BLB）（Xa4，xa5，xa13，Xa21），棕通过标记辅助正向育种（MAFB）方法获得的飞虱（BPH）（Bph3，Bph17），胆mid（GM）（Gm4，Gm8）和QTL（qDTY _1.1和qDTY _3.1）。

结果

在具有干旱QTL的Swarna背景下，采用标记辅助选择（MAS）育种方法，已经开发出七个具有七个到十个基因/ QTL组合的渗入系（IL），用于不同的生物和非生物胁迫。这些IL在农艺性能上均优于各自的轮回亲本，并且具有中等至高直链淀粉含量（AC）（23–26％）的较好谷物品质。其中三个IL，即IL1（Pi9 + Xa4 + xa5 + Xa21 + Bph17 + Gm8 + qDTY _1.1 + qDTY _3.1），IL6（Pi9 + Xa4 + xa5+ Xa21 + Bph3 + Bph17 + Gm4 + Gm8 + qDTY _1.1 + qDTY _3.1）和IL7（Pi9 + Xa4 + xa5 + Bph3 + Gm4 + qDTY _1.1 + qDTY _3.1）在野外和现场均表现出对多种生物和非生物胁迫的抗性\耐受性。温室条件。总体而言，IL在各种胁迫下均具有较高的产量，重要的是，它们在无胁迫条件下也表现良好，且无任何产量损失。

结论

当前的研究清楚地说明了MAS在将多种生物和非生物胁迫的耐受性相结合的同时保持较高的产量潜力和优选的谷物品质的成功。在当前研究中，已开发的具有7至10个基因的ILs在多种生物胁迫（爆炸，BLB，BPH和GM）上表现出优于轮回亲本Swarna +干旱，并且在干旱条件下的产量优势为1.0 t ha ^-1，而没有不利影响在非胁迫下的谷物品质性状。