A short term pot trail was employed to evaluate the exposure of mixed heavy metals (Cu, Pb and Zn) on growth, radial oxygen loss (ROL) and root anatomy in Bruguiera gymnorrhiza. The possible function of BgC4H, a cytochrome P450 gene, on root lignification was also discussed. The exposures of mixed Cu, Pb and Zn directly reduce O₂ leakage at root surface. The reduced ROL inhibited by heavy metals was mainly ascribed by the changes in root anatomical features, such as decreased root porosity together with increased lignification within the exodermis. BgC4H was found to be up-regulated after 0.5-day metal exposure, and remained higher transcript levels within 3-day metal exposure when compared to control roots. Besides, the inhibited photosynthesis may also result in less oxygen can be transported to the underground roots. In summary, the mangrove B. gymnorrhiza appeared to react to external mixed metal contaminants by developing a lignified and impermeable exodermis, and such a root barrier induced by mixed Cu, Pb and Zn appeared to be an adaptive response to block metal ions enters into the roots.

短期盆栽试验被用来评估混合重金属（Cu，Pb和Zn）对布鲁氏裸藻的生长，径向氧损失（ROL）和根部解剖结构的暴露。还讨论了细胞色素P450基因BgC4H对根木质化的可能作用。混合的铜，铅和锌的暴露直接减少了根部表面的O ₂泄漏。重金属抑制的ROL降低主要归因于根部解剖特征的变化，例如根部孔隙率降低以及外皮内木质化程度增加。BgC4H发现与对照根相比，在0.5天金属暴露后，其被上调，并且在3天金属暴露中仍保持较高的转录水平。此外，受抑制的光合作用还可能导致较少的氧气可以被运输到地下根部。综上所述，红树林双歧杆菌似乎通过形成木质素化和不可渗透的外皮而与外部混合金属污染物发生反应，而这种由铜，铅和锌的混合引起的根系屏障似乎是对阻止金属离子进入土壤的适应性反应。根。