The aim of the study was to evaluate oxidant/antioxidant status in 21 Holstein dairy cows with metritis compared to 8 healthy controls. Blood samples were taken during the first 21 days postpartum. Malondialdehyde (MDA), a marker of oxidative stress, total antioxidant status (TAS) and antioxidant parameters such as glutathione peroxidase (GPx), selenium (Se), vitamins A and E and beta-carotene were determined from all cows. The differences in beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), calcium, bilirubin concentrations and aspartate aminotransferase (AST) activity were also monitored, as were milk production and milk composition. Metritis was defined by an unpleasant discharge of varying color (milky-grey/brown/sanguineous) and consistency (muco-purulent/purulent/watery) and by the presence of increased temperature (> 38.5 °C) in cows within 21 days postpartum. Rectal examination revealed increased uterine size, thickened uterine wall and increased uterine tone. The affected cows had significantly reduced daily milk production. Additionally, hematological parameters and haptoglobin concentration were also measured in metritic cows. Higher MDA concentration (P < 0.001) was recorded in cows with metritis, while vitamin A and vitamin E concentrations were lower (P < 0.01) compared to healthy cows. Higher BHB (P < 0.05), NEFA (P < 0.05), AST (P < 0.05) and bilirubin (P < 0.001) concentrations was recorded in cows with metritis as compared to the control group. Significant differences in beta-carotene concentration, GPx activity, and Se, TAS and Ca concentrations in cows with metritis compared to control group were not observed in the present study (P > 0.05). Milk production was decreased in the cows with metritis (P < 0.001) and alterations in milk composition were also observed in metritic cows as compared to healthy cows. The results of the study showed that cows with metritis in early postpartum are exposed to a higher degree of oxidative stress and that the incidence of metritis can negatively affect milk production in dairy cows.

中文翻译：

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评估患有子宫炎的奶牛的氧化剂/抗氧化剂状态、代谢特征和产奶量。

该研究的目的是评估 21 头患有子宫炎的荷斯坦奶牛与 8 头健康对照相比的氧化剂/抗氧化剂状态。在产后的前 21 天采集血样。测定了所有奶牛的氧化应激标志物丙二醛 (MDA)、总抗氧化状态 (TAS) 和抗氧化参数，例如谷胱甘肽过氧化物酶 (GPx)、硒 (Se)、维生素 A 和 E 以及 β-胡萝卜素。还监测了 β-羟基丁酸 (BHB)、非酯化脂肪酸 (NEFA)、钙、胆红素浓度和天冬氨酸氨基转移酶 (AST) 活性的差异，以及牛奶产量和牛奶成分。子宫炎的定义是不同颜色（乳灰色/棕色/血色）和稠度（粘液脓性/脓性/水样）的不愉快分泌物以及温度升高（> 38. 5 °C）在产后 21 天内的奶牛。直肠检查显示子宫增大，子宫壁增厚，子宫张力增加。受影响的奶牛每日产奶量显着减少。此外，还测量了公制奶牛的血液学参数和触珠蛋白浓度。与健康奶牛相比，患有子宫炎的奶牛的 MDA 浓度较高（P < 0.001），而维生素 A 和维生素 E 的浓度较低（P < 0.01）。与对照组相比，患有子宫炎的奶牛的 BHB (P < 0.05)、NEFA (P < 0.05)、AST (P < 0.05) 和胆红素 (P < 0.001) 浓度更高。β-胡萝卜素浓度、GPx 活性和硒的显着差异，与对照组相比，本研究未观察到子宫炎奶牛的 TAS 和 Ca 浓度（P > 0.05）。子宫炎奶牛的产奶量减少（P < 0.001），与健康奶牛相比，子宫炎奶牛的牛奶成分也发生了变化。研究结果表明，产后早期患有子宫炎的奶牛暴露于更高程度的氧化应激，子宫炎的发生会对奶牛的产奶量产生负面影响。