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Transcription factor TGA2 is essential for UV-B stress tolerance controlling oxidative stress in Arabidopsis
bioRxiv - Plant Biology Pub Date : 2020-05-27 , DOI: 10.1101/2020.05.24.113589
Ariel Herrera-Vásquez , Alejandro Fonseca , José Manuel Ugalde , Liliana Lamig , Aldo Seguel , Tomás C. Moyano , Rodrigo A. Gutiérrez , Paula Salinas , Elena A. Vidal , Loreto Holuigue

Plants possess a diversity of Reactive Oxygen Species (ROS)-processing enzymes involved in sensing and controlling ROS levels under basal and stressful conditions. There is little information on the transcriptional regulators that control the expression of these ROS-processing enzymes, particularly at the onset of the defense response to abiotic stress. Filling this gap, this paper reports a critical role for Arabidopsis TGA class II factors (TGA2, TGA5, and TGA6) in the tolerance response to UV-B light and photooxidative stress, by activating the expression of genes with antioxidative roles. We identified two clusters of genes responsive to UV-B and activated by TGA2/5/6 were identified using RNAseq and clustering analysis. The GSTU gene family, which encodes glutathione transferase enzymes from the Tau subclass, was overrepresented in these clusters. We corroborated the TGA2-mediated activation in response to UV-B for three model genes (GSTU7, GSTU8, and GSTU25) using RT-qPCR and ChIP analyses. Interestingly, using tga256 mutant and TGA2- and GSTU7-complemented mutant plants, we demonstrated that TGA2-mediated induction of GSTU genes is essential to control ROS levels and oxidative damage after UV-B and MeV treatments. This evidence positions TGA class II factors, particularly TGA2, as a key players in the redox signaling network of Arabidopsis plants.

中文翻译:

转录因子TGA2对于拟南芥中UV-B胁迫耐受性控制氧化胁迫至关重要

植物具有多种在基础和胁迫条件下参与传感和控制ROS水平的活性氧(ROS)处理酶。关于控制这些ROS处理酶表达的转录调节因子的信息很少,尤其是在对非生物胁迫的防御反应开始时。填补这一空白,本文报道了拟南芥TGA II类因子(TGA2,TGA5和TGA6)在通过激活具有抗氧化作用的基因的表达而对UV-B光和光氧化胁迫的耐受性中的关键作用。我们使用RNAseq和聚类分析确定了对UV-B有响应并被TGA2 / 5/6激活的两个基因簇。GSTU基因家族,它编码来自Tau亚类的谷胱甘肽转移酶,在这些集群中人数过多。我们证实了TGA2介导的针对三个模型基因的UV-B激活(GSTU7GSTU8GSTU25),使用RT-qPCR和ChIP分析。有趣的是,使用tga256突变体以及TGA2GSTU7互补的突变植物,我们证明了TGA2介导的GSTU基因的诱导对于控制ROS水平和UV-B和MeV处理后的氧化损伤至关重要。该证据将TGA II类因子(尤其是TGA2)定位为拟南芥植物氧化还原信号网络中的关键角色。
更新日期:2020-05-27
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