ABSTRACT In this work, we demonstrate, for the first time, a whispering gallery mode (WGM) lasing-based liquid crystal (LC) soft-matter microfiber biosensor for real-time and sensitive detection of hydrogen peroxide and catalase. The functionalised nematic LC 4-cyano-4ʹ-pentylbiphenyl (5CB) microfibers served as both optical microresonators and sensing actuators. The resonance spectral shift in WGMs can be used as a stable and reliable indicator of analyte concentrations. The detection limits for hydrogen peroxide and catalase were as low as ~0.26 μM and ~1 ng/mL, respectively. Direct measurement of the WGM lasing spectrum of LC soft-matter microfibers can be expected as an alternative method to detect analytes without the need for polarised image analysis in current LC sensor systems. This research provides a preliminary basis for the development of soft-matter microfiber biosensors with high sensitivity for detecting biochemical molecules. GRAPHICAL ABSTRACT

中文翻译：

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使用回音壁模式检测过氧化氢和过氧化氢酶的功能化液晶微纤维

摘要 在这项工作中，我们首次展示了一种回音壁模式 (WGM) 基于激光的液晶 (LC) 软物质微纤维生物传感器，用于实时和灵敏地检测过氧化氢和过氧化氢酶。功能化的向列型 LC 4-氰基-4ʹ-戊基联苯 (5CB) 微纤维用作光学微谐振器和传感执行器。WGM 中的共振谱位移可用作分析物浓度的稳定可靠指标。过氧化氢和过氧化氢酶的检测限分别低至 ~0.26 μM 和 ~1 ng/mL。直接测量 LC 软物质微纤维的 WGM 激光光谱有望成为检测分析物的替代方法，而无需在当前的 LC 传感器系统中进行偏振图像分析。该研究为开发高灵敏度的软物质微纤维生物传感器检测生化分子提供了初步基础。图形概要