Abstract The present investigation was accomplished to study the chemical variability of the essential oil of the plant Mentha aquatica L. collected from Bhimtal, Uttarakhand, India during different seasons ranging from April to December. The essential oils collected at various month intervals were subjected to be analyzed with GC along with MS and FID for their comparative chemical composition analysis. Significant differences in the chemical variability were observed in major components of each collection. Linalool represents the dominating compound in every six collections from April to September comprising a total of 30.1 %, 25.2 %, 33.5 %, 48.4 %, 31.1 % and 45.7 % respectively of total oil composition while linalool acetate represents the dominating compound in next three collections from October to December comprising a total of 46.98 %, 42.1 % and 48.0 % respectively of total oil compositions. Thereafter, the essential oil of the tested plant was subjected to assess the efficiency of myorelaxant activity on the isolated duodenum muscles of Swiss Albino mice. Essential oil at different treatments of 2000 μg and 4000 μg resulted into least to the mild grade of relaxation in the muscles evaluated against the maximum response towards acetylcholine, adrenaline, atropine and propanalol.

中文翻译：

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薄荷精油成分的季节性变化及其肌松活性

摘要 本研究旨在研究从印度北阿坎德邦 Bhimtal 采集的水生薄荷精油在 4 月至 12 月不同季节的化学变异性。以不同的月份间隔收集的精油用 GC 以及 MS 和 FID 进行分析，以进行比较化学成分分析。在每个集合的主要成分中观察到化学变异性的显着差异。芳樟醇代表 4 月至 9 月每六个系列中的主要化合物，分别占总油成分的 30.1%、25.2%、33.5%、48.4%、31.1% 和 45.7%，而醋酸芳樟醇代表接下来三个系列的主要化合物从 10 月到 12 月，共占 46.98%，42。分别占总油组合物的 1% 和 48.0%。此后，对受试植物的精油进行评估，对瑞士白化小鼠的十二指肠肌肉进行肌肉松弛活性的效率。根据对乙酰胆碱、肾上腺素、阿托品和普萘洛尔的最大反应评估，2000 μg 和 4000 μg 不同处理的精油导致肌肉松弛程度最低至轻度。