The aim of the present study was to design and fabricate polyglycolic acid (PGA) modules on the basis of the Raschig ring as a tissue element for bottom–top tissue engineering to increase the feasibility of cellular-assembly technology. Three types of modules, namely, cylindrical, Raschig ring, and transverse-pore modules, with different numbers and orientations of canals, were designed and fabricated by modified selective-laser-sintering (SLS) technology. These modules maintained their structure in a flowing culture environment, and degradation did not create an acidic environment, hence promoting their ability to scale up to highly functional tissue. The modules were seeded with human hepatoma Hep G2 cells and cultured for 10 days. The transverse-pore modules were found to have the highest glucose consumption, albumin production, and cell viability among the three tested modules. Our study showed that the proposed module design provided better mass transfer and possessed the required mechanical strength to enable use in the construction of large tissue.

中文翻译：

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在细胞组装技术中以运河为组织元件的聚乙醇酸模块的设计、制造和评估

本研究的目的是设计和制造聚乙醇酸 (PGA) 模块，其基于拉西环作为底部-顶部组织工程的组织元件，以提高细胞组装技术的可行性。采用改进的选择性激光烧结（SLS）技术设计和制造了三种类型的模块，即圆柱形、拉西环和横孔模块，具有不同数量和方向的通道。这些模块在流动的培养环境中保持其结构，降解不会产生酸性环境，因此促进了它们扩展为高功能组织的能力。模块接种人类肝癌 Hep G2 细胞并培养 10 天。发现横孔模块具有最高的葡萄糖消耗、白蛋白产量、和三个测试模块之间的细胞活力。我们的研究表明，所提出的模块设计提供了更好的传质，并具有所需的机械强度，可用于构建大型组织。