Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.

中文翻译：

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苦参愈伤组织培养物中黄酮的产生。

黄酮类化合物，包括苦参（Sophora flavescens Aiton）根中的maackiain（Maac），具有许多药理特性，例如抗肿瘤，抗微生物和抗真菌活性。这项研究的目的是开发一种用于苦参的体外植物和愈伤组织培养系统，以期产生一种替代的生产系统，以增强苦参的生产，因为通常在苦参的根中存在少量的Maac 。我们安排了苦参不同组织的最佳条件并在培养基中添加了各种植物生长调节剂（PGR）。在所有浓度的噻唑隆（TDZ）和吡咯烷的联合处理中，愈伤组织的诱导和增殖率最高。此外，在Murashige和Skoog（MS）培养基中，在2.0 mg / L吡咯烷和0.5 mg / L 6-苄基腺嘌呤（BA）上培养的叶片外植体诱导的愈伤组织具有最高的活性代谢产物Maac积累。在含有TDZ和α-萘乙酸（NAA）组合的培养基上再生体外芽。成功地建立了使用苦参愈伤组织培养物大规模生产次级代谢产物的可靠方案。