Bak-ri-hyang (Thymus quinquecostatus Celak.) is an important medicinal and aromatic plant in Korea. T. quinquecostatus population and is always mixed with other thyme cultivars during cultivation and marketing. Hence, this study aimed to determine the genetic variability and the essential oil composition of three Korean native thyme, T. quinquecostatus cultivars collected from the Wolchul, Jiri, and Odae mountains, in comparison with six commercial thyme cultivars (T. vulgaris), to distinguish Bak-ri-hyang from other thyme cultivars. The composition of essential oils obtained from nine individuals was analyzed by gas chromatography–mass spectrometry (GC–MS). The random amplified polymorphic DNA (RAPD) analysis was accomplished using 16 different primers. The GC–MS analysis revealed that Wolchul, creeping, golden, and orange cultivars belong to the geraniol chemotype. Whereas the Odae, lemon, and silver cultivars belong to the thymol chemotype. Further, linalool was the most abundant component in carpet and Jiri cultivars. The RAPD analysis demonstrated that all thyme cultivars showed characteristic RAPD patterns that allowed their identification. In total, 133 bands were obtained using 16 primers, and 124 bands were polymorphic, corresponding to 93.2% polymorphism. Cluster analysis of RAPD markers established the presence of clear separation from nine thyme cultivars. The highest dissimilarity and similarity coefficient of the RAPD markers were 0.58 and 0.98, respectively. According to the RAPD patterns, the nine thyme cultivars could be divided into two major clusters. Among three Korean cultivars, the Wolchul and Odae cultivars were placed into the same cluster, but they did not show identical clustering with their essential oil compositions. The findings of the present study suggest that RAPD analysis can be a useful tool for marker-assisted identification of T. quinquecostatus from other Thymus species.

中文翻译：

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韩国本土百里香Bak-Ri-Hyang（Thymus quinquecostatus Celak。）的形态，化学和遗传特征。

百里香（Thymus quinquecostatus Celak。）是韩国重要的药用和芳香植物。T. quinquecostatus种群，在种植和销售期间总是与其他百里香品种混合在一起。因此，本研究旨在确定与六个商业百里香品种（T. vulgaris）相比，从Wolchul，Jiri和Odae山区收集的三种韩国本土百里香T. quinquecostatus品种的遗传变异性和精油成分。），以将Bak-ri-hyang与其他百里香品种区分开来。通过气相色谱-质谱法（GC-MS）分析了从9个人获得的精油成分。使用16种不同引物完成了随机扩增多态性DNA（RAPD）分析。GC-MS分析表明，Wolchul，creep，金色和橙色品种属于香叶醇的化学型。而Odae，柠檬和银品种属于百里酚化学型。此外，芳樟醇是地毯和吉里品种中含量最丰富的成分。RAPD分析表明，所有百里香品种均表现出特征性的RAPD模式，可以对其进行鉴定。总共，使用16个引物获得133条带，并且124条带是多态性的，对应于93.2％的多态性。RAPD标记的聚类分析确定了与9个百里香品种的明确分离。RAPD标记的最高相似度和相似系数分别为0.58和0.98。根据RAPD模式，可以将9个百里香品种分为两个主要类群。在三个韩国品种中，Wolchul和Odae品种被放在同一群中，但是它们的精油成分没有表现出相同的群。本研究的结果表明，RAPD分析可以作为标记物辅助鉴定的有用工具。九个百里香品种可分为两个主要类群。在三个韩国品种中，Wolchul和Odae品种被放置在同一簇中，但是它们的精油成分并没有表现出相同的簇。本研究的结果表明，RAPD分析可以作为标记物辅助鉴定的有用工具。九个百里香品种可分为两个主要类群。在三个韩国品种中，Wolchul和Odae品种被放置在同一簇中，但是它们的精油成分并没有表现出相同的簇。本研究的发现表明，RAPD分析可以作为标记物辅助鉴定的有用工具。其他百里香属的T. quinquecostatus。