We demonstrate a reconnectable implantable ultraslim fiber‐optic microendoscope that integrates a branching fiber bundle (BFB) with gradient‐index fiber lenses, enabling a simultaneous fluorescence imaging of individual cells in distinctly separate brain regions, including brain structures as distant as the neocortex and hippocampus. We show that fluorescence images of individual calcium‐indicator‐expressing neurons in the brain of freely moving transgenic mice can be recorded, via the implanted BFB probe, in parallel with time‐ and cell‐resolved traces of calcium signaling, thus enabling correlated circuit‐dynamics studies at ‐multiple sites within the brain of freely moving animals.

中文翻译：

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多部位细胞和神经动力学解析深大脑成像，植入可重新连接的纤维束的自由移动小鼠。

我们展示了一种可重新连接的植入式超薄光纤微内窥镜，该微内窥镜将分支纤维束（BFB）与梯度折射率纤维透镜集成在一起，从而可以对分别明显的大脑区域（包括远至新皮层和海马体的大脑结构）中的单个细胞进行同步荧光成像。我们显示，通过植入的BFB探针可以记录自由移动的转基因小鼠大脑中表达钙指示剂的单个神经元的荧光图像，同时记录钙信号的时间和细胞解析轨迹，从而实现相关的电路在自由移动的动物的大脑内多个部位进行动力学研究。