Detection and isolation of the typical gut indigenous bacteria from ddY mice fed a casein-beef tallow-based or egg yolk-based diet.,Journal of Food Biochemistry

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Detection and isolation of the typical gut indigenous bacteria from ddY mice fed a casein-beef tallow-based or egg yolk-based diet.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-05-27 , DOI: 10.1111/jfbc.13246
Mayu Fukunaga ₁ , Takashi Kuda ₁ , Yumeng Xia ₁ , Saori Nakamura ₁ , Hajime Takahashi ₁ , Bon Kimura ₁

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The effects of whole egg on the cecal microbiome of ddY mice has been reported. To investigate the existence of susceptible indigenous bacteria (SIB) to egg yolks (EY), mice were fed a diet containing either 20% (w/w) milk casein and 17% beef tallow (CT) or 12% milk casein and 27% EY for 14 days, and then, the cecal microbiome was analyzed by 16S rRNA (V4) amplicon sequencing. To isolate the typical species in each diet group, culture‐dependent viable bacterial counts were determined on Blood Liver (BL) and Gifu Anaerobic Medium (GAM) agar plates. The amplicon sequencing analysis revealed typical CT‐SIB, such as Lachnospiraceae‐like bacteria, and EY‐SIB, such as Allobaculum‐, Lactobacillus murinus‐, and Bacteroides vulgatus‐like bacteria. Two of the detected SIB species, L. murinus‐ and B. vulgatus‐like bacteria, were successfully isolated from the BL and GAM agar plates and defined using a 16S rDNA BLAST search.

中文翻译：

从以酪蛋白牛肉牛脂或蛋黄为基础饮食的ddY小鼠中检测和分离出典型的肠道固有细菌。

已经报道了全卵对ddY小鼠的盲肠微生物组的影响。为了研究对蛋黄（EY）敏感的本地细菌（SIB）的存在，给小鼠喂食了一种饮食，该饮食含有20％（w / w）的牛奶酪蛋白和17％的牛脂（CT）或12％的牛奶酪蛋白和27％ EY处理14天，然后通过16S rRNA（V4）扩增子测序分析盲肠微生物组。为了分离每个饮食组中的典型物种，在血肝（BL）和岐阜厌氧培养基（GAM）琼脂平板上测定了依赖培养物的存活细菌数。扩增子测序分析显示典型的CT-SIB，如毛螺-如细菌，和EY-SIB，如Allobaculum - ，乳杆菌鼱- ，和拟杆菌普通拟类细菌。从BL和GAM琼脂平板上成功分离出两种检测到的SIB物种，即鼠李斯特氏菌和寻常型B. vulgatus细菌，并使用16S rDNA BLAST搜索对其进行了定义。

更新日期：2020-05-27

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