This study used microbial rDNA sequencing for accurate detection of systemic lupus erythematosus (SLE) skin lesions. 20 lupus erythematosus dermatology patients and 20 healthy persons were selected as experimental group and control group. Feces and serum of the subjects were sampled in sterile environment. Serum samples were examined for anti dsDNA antibodies. Fecal samples were analyzed by 16 s rDNA high-throughput sequencing, using Illumina MiSeq 2 × 250 sequencing platform. The results suggested that positive rate of anti-dsDNA antibody in serum was significantly higher in the experimental group than the control group. Significant difference of intestinal microbiome was spotted between the two groups in phylum (Firmicutes, Bacteroidetes) and genus level (Lactobacillus, Allobaculum, Lachnospira, Turicibacter, Bifidobacterium). The different intestinal microbiomes existing in healthy people and patients can provide a strong tool for accurate diagnose of lupus erythematosus.

这项研究使用微生物rDNA测序来准确检测系统性红斑狼疮（SLE）皮肤病变。选择20例红斑狼疮皮肤病患者和20例健康人作为实验组和对照组。在无菌环境中对受试者的粪便和血清进行采样。检查血清样品中的抗dsDNA抗体。使用Illumina MiSeq 2×250测序平台，通过16 s rDNA高通量测序分析粪便样品。结果表明，实验组血清中抗dsDNA抗体的阳性率明显高于对照组。两组的肠道（菌毛，拟杆菌）和属水平（乳杆菌）之间均存在明显差异。，同种异体菌，拉克螺旋菌，鼻菌，双歧杆菌）。健康人和患者中存在的不同肠道微生物群可以为准确诊断红斑狼疮提供强有力的工具。