This paper represents a comprehensive study of two new thraustochytrids and a marine Rhodotorula red yeast isolated from Australian coastal waters for their abilities to be a potential renewable feedstock for the nutraceutical, food, fishery and bioenergy industries. Mixotrophic growth of these species was assessed in the presence of different carbon sources: glycerol, glucose, fructose, galactose, xylose, and sucrose, starch, cellulose, malt extract, and potato peels. Up to 14 g DW/L (4.6 gDW/L-day and 2.8 gDW/L-day) of biomass were produced by Aurantiochytrium and Thraustochytrium species, respectively. Thraustochytrids biomass contained up to 33% DW of lipids, rich in omega-3 polyunsaturated docosahexaenoic acid (C22:6, 124 mg/g DW); up to 10.2 mg/gDW of squalene and up to 61 μg/gDW of total carotenoids, composed of astaxanthin, canthaxanthin, echinenone, and β-carotene. Along with the accumulation of these added-value chemicals in biomass, thraustochytrid representatives showed the ability to secrete extracellular polysaccharide matrixes containing lipids and proteins. Rhodotorula sp lipids (26% DW) were enriched in palmitic acid (C16:0, 18 mg/gDW) and oleic acid (C18:1, 41 mg/gDW). Carotenoids (87 μg/gDW) were mainly represented by β-carotene (up to 54 μg/gDW). Efficient growth on organic and inorganic sources of carbon and nitrogen from natural and anthropogenic wastewater pollutants along with intracellular and extracellular production of valuable nutrients makes the production of valuable chemicals from isolated species economical and sustainable.

本文代表了对两种新的破囊壶菌和从澳大利亚沿海水域分离出的海洋红酵母红酵母的全面研究，这些酵母具有作为营养食品，食品，渔业和生物能源行业的潜在可再生原料的能力。在不同碳源的存在下，评估了这些物种的混合营养生长：甘油，葡萄糖，果糖，半乳糖，木糖和蔗糖，淀粉，纤维素，麦芽提取物和马铃薯皮。最多至14g DW / L（4.6 GDW / L-天和2.8 GDW / L天）生物质被产生的Aurantiochytrium和破囊壶菌种类。破囊壶菌的生物量含有高达33％的DW脂质，富含omega-3多不饱和二十二碳六烯酸（C22：6，124 mg / g DW）；高达10.2 mg / gDW的角鲨烯和高达61μg/ gDW的总类胡萝卜素，由虾青素，角黄素，海胆烯酮和β-胡萝卜素组成。随着这些增值化学品在生物质中的积累，破囊壶菌的代表表现出了分泌包含脂质和蛋白质的细胞外多糖基质的能力。红杜鹃sp脂质（26％DW）富含棕榈酸（C16：0，18 mg / gDW）和油酸（C18：1，41 mg / gDW）。类胡萝卜素（87μg/ gDW）主要由β-胡萝卜素（高达54μg/ gDW）表示。来自自然和人为废水污染物的有机和无机碳和氮源的有效生长，以及细胞内和细胞外有价值营养素的产生，使得从孤立物种生产有价值的化学物质经济且可持续。