There is growing evidence that some parasitic infections can impact a variety of autoimmune diseases by disease-inducing or protecting capacities. Anti-inflammatory molecules secreted by Toxoplasma gondii and other parasites are capable of preventing some autoimmune disease like arthritis, lupus nephritis and systemic lupus erythematosus by acting on the immune system. Here we aimed to evaluate the protective efficacy of vaccination with Toxoplasma gondii (T. gondii), either gamma radiation-attenuated or not, on an adjuvant arthritis mouse model. Forty female Swiss albino mice were conducted in experiment divided into normal control; mice were injected with Complete Freund's adjuvant (CFA) into the right hind paws; mice vaccinated with irradiated T. gondii in the 3rd group and un-irradiated T. gondii in the 4th group then were injected two weeks later with CFA. Histopathological changes and IL-17, STAT6 and ROR-γ levels in the joints, as well as serum survivin and Anti-CCP, were evaluated. Also, the splenic production of TGF-β1, TGF-βR, IL-23, IL-1β, IFN-γ, TFN-∞, NF_Kβ, MMP1 and MMP3 were assessed. Results exhibited an enhancement of the histopathological changes with diminished the rise of IL-17, STAT 6 and ROR- γ within the joints of both vaccinated groups. Also, serum survivin and Anti-CCP, as well as splenic inflammatory cytokines were reduced. It can be concluded that vaccination with un-irradiated or radiation-attenuated T. gondii exerted a protective effect against adjuvant arthritis with better pathological achievement in the radiation-attenuated vaccinated group. Using gamma radiation-attenuated parasite to exclude the delirious effects of imposing infection of live one may pave the way to new preventative modality against rheumatoid arthritis.

越来越多的证据表明，某些寄生虫感染可通过诱发或保护疾病的能力来影响多种自身免疫性疾病。弓形虫和其他寄生虫分泌的抗炎分子能够通过作用于免疫系统来预防某些自身免疫性疾病，例如关节炎，狼疮肾炎和系统性红斑狼疮。在这里，我们旨在评估佐剂弓形虫（T. gondii）的疫苗接种对佐剂性关节炎小鼠模型的保护作用，无论γ射线辐射减弱与否。实验进行了40只雌性瑞士白化病小鼠，分为正常对照组；给小鼠右后爪注射完全弗氏佐剂（CFA）；用辐照疫苗接种的小鼠然后，在第3组的刚地弓形虫和在第4组的未辐照的刚地弓形虫在两周后注射CFA。评价了关节的组织病理学变化和IL-17，STAT6和ROR-γ水平，以及血清survivin和抗CCP。另外，脾生产TGF-β1，TGF-βR，IL-23，IL-1β，IFN-γ的，TFN-∞，NF _ķ β，MMP1及MMP3进行了评估。结果显示，两个疫苗接种组的关节内IL-17，STAT 6和ROR-γ的升高均降低了组织病理学变化。而且，血清存活蛋白和抗CCP以及脾炎性细胞因子也降低了。可以得出结论，用未辐照或辐射减弱的弓形虫进行疫苗接种减毒的疫苗接种组对佐剂性关节炎具有保护作用，并具有更好的病理学效果。使用伽玛射线衰减的寄生虫来排除强加给活人的感染所带来的烦恼效应，可能会为风湿性关节炎的新预防方式铺平道路。