Background

p-Coumaric acid (p-CA), a phenolic compound abundantly found in edible plants, was found to induce apoptosis in SNU-16 gastric cancer cells. However, the effects of p-CA on the microRNA expression pattern in SNU-16 cells have not been reported.

Objectives

The primary objective of this study was to investigate microRNA expression profiles in p-CA-treated SNU-16 cells.

Methods

SNU-16 cells were treated with 1.5 mM p-CA for 48 h. High-throughput RNA sequencing was performed. The accuracy and validity of the RNA sequencing results were tested by real-time PCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the differentially expressed microRNAs (DEMs).

Results

Thirty-two DEMs were identified between p-CA-treated and control cells, 9 of which were upregulated, and 23 downregulated. GO and KEGG pathway analyses of the target genes indicated that these genes were involved in a variety of biological functions and several cancer-related pathways. The miRNA interactive network analysis identified hsa-miR-125a-5p, hsa-miR-30a-5p, and hsa-miR-7-5p as the major nodes.

Conclusions

Our findings indicated that p-CA exerted its anticancer effects in SNU-16 gastric cancer cells by modulating the expression of certain microRNAs, providing a better understanding of the anticancer properties of p-CA in gastric cancer cells.

中文翻译：

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对香豆酸对SNU-16人胃癌细胞microRNA表达谱的影响。

背景

p香豆酸（p -CA），在食用植物大量存在的酚类化合物，发现诱导凋亡在SNU-16胃癌细胞。然而，尚未报道p- CA对SNU-16细胞中的微小RNA表达模式的影响。

目标

这项研究的主要目的是研究经p -CA处理的SNU-16细胞中的microRNA表达谱。

方法

用1.5 mM p -CA处理SNU-16细胞48小时。进行高通量RNA测序。RNA测序结果的准确性和有效性通过实时PCR进行了测试。使用差异表达的microRNA（DEM）进行了基因本体论（GO）富集和《京都议定书》的基因与基因组百科全书（KEGG）途径分析。

结果

在经p -CA处理的细胞和对照细胞之间鉴定出32个DEM ，其中9个被上调，而23个被下调。对靶基因的GO和KEGG途径分析表明，这些基因参与了多种生物学功能和几种与癌症相关的途径。miRNA交互式网络分析确定了hsa-miR-125a-5p，hsa-miR-30a-5p和hsa-miR-7-5p是主要节点。

结论

我们的发现表明，p -CA通过调节某些microRNA的表达在SNU-16胃癌细胞中发挥其抗癌作用，从而更好地了解p -CA在胃癌细胞中的抗癌特性。