Genes & Genomics ( IF 1.6 ) Pub Date : 2020-05-27 , DOI: 10.1007/s13258-020-00944-6 Mi Gyeong Jang 1, 2 , Hee Chul Ko 2 , Se-Jae Kim 1, 2
Background
p-Coumaric acid (p-CA), a phenolic compound abundantly found in edible plants, was found to induce apoptosis in SNU-16 gastric cancer cells. However, the effects of p-CA on the microRNA expression pattern in SNU-16 cells have not been reported.
Objectives
The primary objective of this study was to investigate microRNA expression profiles in p-CA-treated SNU-16 cells.
Methods
SNU-16 cells were treated with 1.5 mM p-CA for 48 h. High-throughput RNA sequencing was performed. The accuracy and validity of the RNA sequencing results were tested by real-time PCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the differentially expressed microRNAs (DEMs).
Results
Thirty-two DEMs were identified between p-CA-treated and control cells, 9 of which were upregulated, and 23 downregulated. GO and KEGG pathway analyses of the target genes indicated that these genes were involved in a variety of biological functions and several cancer-related pathways. The miRNA interactive network analysis identified hsa-miR-125a-5p, hsa-miR-30a-5p, and hsa-miR-7-5p as the major nodes.
Conclusions
Our findings indicated that p-CA exerted its anticancer effects in SNU-16 gastric cancer cells by modulating the expression of certain microRNAs, providing a better understanding of the anticancer properties of p-CA in gastric cancer cells.
中文翻译:
对香豆酸对SNU-16人胃癌细胞microRNA表达谱的影响。
背景
p香豆酸(p -CA),在食用植物大量存在的酚类化合物,发现诱导凋亡在SNU-16胃癌细胞。然而,尚未报道p- CA对SNU-16细胞中的微小RNA表达模式的影响。
目标
这项研究的主要目的是研究经p -CA处理的SNU-16细胞中的microRNA表达谱。
方法
用1.5 mM p -CA处理SNU-16细胞48小时。进行高通量RNA测序。RNA测序结果的准确性和有效性通过实时PCR进行了测试。使用差异表达的microRNA(DEM)进行了基因本体论(GO)富集和《京都议定书》的基因与基因组百科全书(KEGG)途径分析。
结果
在经p -CA处理的细胞和对照细胞之间鉴定出32个DEM ,其中9个被上调,而23个被下调。对靶基因的GO和KEGG途径分析表明,这些基因参与了多种生物学功能和几种与癌症相关的途径。miRNA交互式网络分析确定了hsa-miR-125a-5p,hsa-miR-30a-5p和hsa-miR-7-5p是主要节点。
结论
我们的发现表明,p -CA通过调节某些microRNA的表达在SNU-16胃癌细胞中发挥其抗癌作用,从而更好地了解p -CA在胃癌细胞中的抗癌特性。