The effectiveness of two different rapid methods involving the 3M™ molecular detection assay Listeria and the 3M™ Petrifilm environmental Listeria Plate were evaluated for the rapid detection of Listeria from naturally contaminated vegetables and chicken-processing environments against the standard culture-based method. A total of 178 samples were examined for the presence of Listeria. A total of 47/178 (26.4%) by standard ISO culture-based method (EN ISO 11290-1), 42/178 (23.6%) by 3M™ MDA Listeria and 40/178 (22.5%) by 3M™ Petrifilm EL Plate showed positive results, respectively. The accuracy, sensitivity, specificity, positive predictive value, and negative predictive value for 3M™ MDA Listeria and 3M™ Petrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and 96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on the Cohen’s Kappa value, there was a complete and robust concordance between 3M™ MDA Listeria (0.911) and 3M™ Petrifilm EL Plates (0.894) as compared to the standard culture-based method.

评估了两种不同的快速方法的有效性，这些方法涉及3M™分子检测利斯特氏菌和3M™Petrifilm环境利斯特氏菌平板，用于针对自然污染蔬菜和鸡肉加工环境中基于标准培养方法的利斯特氏菌的快速检测。总共检查了178个样品中是否存在李斯特菌。通过基于ISO文化的标准方法（EN ISO 11290-1）总计47/178（26.4％），通过3M™MDA李斯特菌获得的42/178（23.6％）和通过3M™Petrifilm EL获得的40/178（22.5％）平板分别显示阳性结果。3M™MDA的准确性，敏感性，特异性，阳性预测值和阴性预测值李斯特菌和3M™Petrifilm EL板分别为97.2％，89.4、99.3、97.7、96.4％和96.1、85.1、100.0、100.0、94.9％。基于Cohen的Kappa值，与基于标准培养的方法相比，3M™MDA李斯特菌（0.911）和3M™Petrifilm EL板（0.894）之间具有完全而稳健的一致性。