Polyurethane (PU) elastomers were synthesized by the reaction of HDI or IPDI diisocyanates and poly(ε-caprolactone) (PCL or poly(ethylene adipate) (PA) diols and ethylene glycol as a polymer chain extender. IR, 1 H, and 13 C NMR spectroscopy and X-ray analysis were used for the structural analysis of the formed films. The molecular weight distribution was examined by GPC chromatography. Based on the measured contact angles, free surface energy parameters were calculated. The obtained results were analyzed for the possible use of these polyurethanes as biomaterials. The most promising in this respect was PU-3, which was synthesized from IPDI and PCL. This was due to its high molecular weight of approximately 90,000, the presence of a crystalline phase, and the relatively high hydrophobicity, with a SEP value below 25 mJ/m 2 . These films showed a good resistance to hydrolysis during incubation in Baxter physiological saline during 6 weeks. Both Gram-positive ( Bacillus sp.) and Gram-negative ( Pseudomonas sp.) types of bacterial strains were used to test the biodegradation property. Synthesized PUs are biodegradable and showed moderate or even mild cytotoxicity against human normal fibroblasts (BJ) and immortalized keratinocytes (HaCaT), estimated with direct contact assay. The most biocompatible was PU-3 film, which revealed rather mild reactivity against both cell lines, and the least was PU-2 film, synthesized from HDI and PA (severe toxicity for HaCaTs).

中文翻译：

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基于非芳族异氰酸酯、聚酯和乙二醇的生物医学应用聚氨酯弹性体的合成和性能

这些薄膜在 Baxter 生理盐水中孵育 6 周期间显示出良好的耐水解性。使用革兰氏阳性（芽孢杆菌属）和革兰氏阴性（假单胞菌属）类型的细菌菌株来测试生物降解特性。合成的 PU 是可生物降解的，通过直接接触试验估计，对人类正常成纤维细胞 (BJ) 和永生化角质形成细胞 (HaCaT) 显示出中等甚至轻微的细胞毒性。生物相容性最好的是 PU-3 薄膜，它对两种细胞系都表现出相当温和的反应性，最少的是由 HDI 和 PA 合成的 PU-2 薄膜（对 HaCaT 具有严重的毒性）。) 类型的细菌菌株用于测试生物降解性能。合成的 PU 是可生物降解的，通过直接接触试验估计，对人类正常成纤维细胞 (BJ) 和永生化角质形成细胞 (HaCaT) 显示出中等甚至轻微的细胞毒性。生物相容性最好的是 PU-3 薄膜，它对两种细胞系都表现出相当温和的反应性，最少的是由 HDI 和 PA 合成的 PU-2 薄膜（对 HaCaT 具有严重的毒性）。) 类型的细菌菌株用于测试生物降解性能。合成的 PU 是可生物降解的，通过直接接触试验估计，对人类正常成纤维细胞 (BJ) 和永生化角质形成细胞 (HaCaT) 显示出中等甚至轻微的细胞毒性。生物相容性最好的是 PU-3 薄膜，它对两种细胞系都表现出相当温和的反应性，最少的是由 HDI 和 PA 合成的 PU-2 薄膜（对 HaCaT 具有严重的毒性）。