Tephrosia, an important medicinal plant, and a potential livestock feed, was found to be affected by a leaf yellowing disease at the Ziway field site of the International Livestock Research Institute. A total of fifty samples from 300 plants were collected from twenty Tephrosia species in three consecutive planting seasons; 2015, 2016 and 2017. The samples were screened for viral infection by dot-blot assay with antiserum targetting eight viruses. RT-PCR of dot-blot positive samples using virus specific primers gave an amplification product of the expected size (867 bp) only for cucumber mosaic virus (CMV) in Tephrosia senna . The amplified products were sequenced; coat protein sequence (657 bp) extracted, and submitted to NCBI database (Tep-Et; KY041651). Sequence alignment and phylogenetic analyses indicated that the isolate, Tep-Et, shared maximum identity [88.8–97.5% nucleotide (nt) and 89.4–96.3% amino acid (aa)] with CMV belonging to members of subgroup-I. To our knowledge, this is the first report of molecular characterisation of a CMV isolate infecting a new host, T. senna in Ethiopia.

中文翻译：

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埃塞俄比亚毛茛黄叶病病毒的筛选与鉴定

在国际畜牧研究所 Ziway 现场发现，一种重要的药用植物和一种潜在的牲畜饲料——毛茛受到叶片黄化病的影响。在连续三个种植季节从 20 个 Tephrosia 物种中收集了 300 株植物的 50 个样品；2015 年、2016 年和 2017 年。通过斑点印迹法使用针对八种病毒的抗血清对样本进行病毒感染筛查。使用病毒特异性引物对斑点印迹阳性样品进行 RT-PCR 得到预期大小 (867 bp) 的扩增产物，仅适用于番泻叶中的黄瓜花叶病毒 (CMV)。对扩增产物进行测序；外壳蛋白序列 (657 bp) 提取，并提交给 NCBI 数据库 (Tep-Et; KY041651)。序列比对和系统发育分析表明分离株 Tep-Et，与属于亚组 I 成员的 CMV 共享最大同一性 [88.8-97.5% 核苷酸 (nt) 和 89.4-96.3% 氨基酸 (aa)]。据我们所知，这是对感染新宿主 T. senna 在埃塞俄比亚的 CMV 分离株进行分子表征的第一份报告。