Indigenous entomopathogenic nematode, Heterorhabditis pakistanensis, NBAIR H-05 strain was evaluated against diapausing larvae of the Codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae) at 3 different dosages, i.e., at 15, 20, and 25 gm/ l of water in apple orchards at the district Kargil of Ladakh Region, India, during 2017 and 2018.Two year’s pooled average density of diapausing ranged 34.6 to 56.8 larvae/trunk band before treatment, which declined ranging 43.85 to 86.27 % with respect to different treatments of entomopathogenic nematode at concentrations between 7.5 × 105 IJs to 1.25 × 106 IJs/tree. Percent reduction in larvae over control varied from 41.78 to 85.77% for 7.5 × 105 IJs and 1.25 × 106 IJs respectively. Two-year pooled data indicated larval mortality between 39.85 and 73.38% and 4.0 to 12.89% with respect to different treatments at 48 and 72 h respectively, with statistically significant difference (P = ˂ 0.001). Increase in dosage of nematode formulation from 15 gm to 25 gm resulted in increased larval mortality (r = 0.92**). Post wetting of trunk band after 24 h in each treatment resulted in significantly higher larval mortality than non-post wetting. There was non-significant difference (t = 0.83) between larval mortality with respect to treatments during 2017 and 2018.

中文翻译：

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在拉达克地区苹果种植的丘陵地带，昆虫病原线虫的本地菌株对苹果蛾蛾（Cydia pomonella L.（鳞翅目：Tortricidae））的滞育幼虫的杀灭作用

以3种不同剂量（即15、20和25 gm / l的水）评估了土著昆虫病原线虫，巴基斯坦异种虫线虫，NBAIR H-05菌株对di蛾蛾（Cydia pomonella L.（鳞翅目：Tortricidae））的滞育幼虫的滞育性2017年至2018年期间，印度拉达克地区卡吉尔地区的苹果园中，平均滞育的合并平均滞育密度为34.6至56.8个幼虫/树干，处理前降低了43.85％至86.27％浓度在7.5×105 IJs至1.25×106 IJs /棵之间的线虫。对于7.5×105 IJ和1.25×106 IJ，幼虫对控制的减少百分比分别从41.78到85.77％。两年汇总数据显示，幼虫死亡率在39.85％至73.38％之间以及4.0至12岁之间。分别在48和72 h时，不同治疗的比例为89％，差异有统计学意义（P = 0.001）。线虫制剂的剂量从15 gm增加到25 gm导致幼虫死亡率增加（r = 0.92 **）。每次处理后24小时，躯干带的后润湿比未后润湿导致幼虫死亡率显着提高。2017年和2018年间，幼虫死亡率与治疗之间无显着差异（t = 0.83）。