The YEATS domain is a highly conserved protein structure that interacts with acetylated and crotonylated lysine residues in N-terminal tails of histones. The budding yeast genome encodes three YEATS domain proteins (Taf14, Yaf9, and Sas5) that are all the subunits of different complexes involved in histone acetylation, gene transcription, and chromatin remodeling. As the strains deficient in all these three genes are inviable, it has been proposed that the YEATS domain is essential in yeast. In this study we investigate in more detail the requirement of YEATS domain proteins for yeast survival and the possible roles of Taf14 YEATS domain in the regulation of gene transcription. We found that YEATS domains are not essential for the survival of Saccharomyces cerevisiae cells. Although the full deletion of all YEATS proteins is lethal in yeast, we show that the viability of cells can be restored by the expression of the YEATS-less version of Taf14 protein. We also explore the in vivo functions of Taf14 protein and show that the primary role of its YEATS domain is to stabilize the transcription pre-initiation complex (PIC). Our results indicate that Taf14-mediated interactions become crucial for PIC formation in rpb9Δ cells, where the recruitment of TFIIF to the PIC is hampered. Although H3 K9 residue has been identified as the interaction site of the Taf14 YEATS domain in vitro, we found that it is not the only interaction target in vivo. Lethality of YEATS-deficient cells can be rescued by the expression of truncated Taf14 protein lacking the entire YEATS domain, indicating that the YEATS domains are not required for cell survival. The YEATS domain of Taf14 participates in PIC stabilization and acetylated/crotonylated H3K9 is not the critical target of the Taf14 YEATS domain in vivo.

中文翻译：

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Taf14是稳定酿酒酵母中转录前起始复合物所必需的。

YEATS结构域是高度保守的蛋白质结构，可与组蛋白N末端尾巴中的乙酰化和巴豆酰化的赖氨酸残基相互作用。出芽的酵母基因组编码三个YEATS域蛋白（Taf14，Yaf9和Sas5），它们是参与组蛋白乙酰化，基因转录和染色质重塑的不同复合物的所有亚基。由于在这三个基因中都缺乏的菌株是不能存活的，因此提出YEATS结构域在酵母中是必不可少的。在这项研究中，我们将更详细地研究酵母菌生存所需的YEATS结构域蛋白以及Taf14 YEATS结构域在基因转录调控中的可能作用。我们发现，YEATS域对于酿酒酵母细胞的生存不是必需的。尽管酵母中所有YEATS蛋白的完全删除是致命的，我们表明，可以通过少表达YEATS的Taf14蛋白的表达来恢复细胞的活力。我们还探讨了Taf14蛋白的体内功能，并表明其YEATS域的主要作用是稳定转录前起始复合物（PIC）。我们的结果表明，Taf14介导的相互作用对于rpb9Δ细胞中PIC的形成至关重要，其中TFIIF向PIC的募集受到阻碍。尽管H3 K9残基已被确定为Taf14 YEATS域在体外的相互作用位点，但我们发现它不是体内唯一的相互作用靶标。缺乏完整的YEATS结构域的截短的Taf14蛋白的表达可以挽救缺乏YEATS的细胞的死亡，这表明细胞存活不需要YEATS结构域。