Emerging technologies for profiling extracellular vesicle heterogeneity.,Lab on a Chip

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Emerging technologies for profiling extracellular vesicle heterogeneity.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-05-27 , DOI: 10.1039/d0lc00431f
Guan Huang ₁ , Gungun Lin , Ying Zhu , Wei Duan , Dayong Jin

Affiliation

Extracellular vesicles (EVs) are membrane-bound vesicles secreted by most cell types and exist in virtually all bodily fluids. They carry on a wealth of proteomic and genetic information including proteins, lipids, miRNAs, mRNA, non-coding RNA and other molecules from parental cells. Increasing evidence shows that within populations of EVs, their biogenesis, physical characteristics (e.g. size, density, morphology) and cargos (e.g. protein, lipid content, nucleic acids) may vary substantially, which accordingly change their biological properties. To fully exploit the potential of EVs, it requires qualified methods to profile EV heterogeneity. In this review, we survey recent approaches for EV isolation with innovative discoveries in heterogeneity. The main challenges in EV heterogeneity research are identified, and the roles of single cell EV profiling and single EV imaging are highlighted. We further discuss promising opportunities for resolving the underlying complexity of EV heterogeneity.

中文翻译：

分析细胞外囊泡异质性的新兴技术。

细胞外囊泡（EVs）是大多数细胞类型分泌的膜结合囊泡，实际上存在于所有体液中。它们具有大量的蛋白质组学和遗传信息，包括蛋白质，脂质，miRNA，mRNA，非编码RNA和其他来自亲代细胞的分子。越来越多的证据表明，在电动汽车群体中，它们的生物发生，物理特征（例如大小，密度，形态）和货物（例如蛋白质，脂质含量，核酸）可能会发生很大变化，从而相应地改变其生物学特性。为了充分利用电动汽车的潜力，它需要合格的方法来描述电动汽车的异质性。在这篇综述中，我们调查了具有异质性创新发现的最新电动汽车隔离方法。确定了电动汽车异质性研究中的主要挑战，并重点介绍了单细胞电动汽车轮廓分析和单电动汽车成像的作用。我们将进一步讨论解决EV异质性潜在复杂性的有前途的机会。

更新日期：2020-07-14

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