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Diuretic and Renal Protective Effect of Kaempferol 3-O-Alpha-l-rhamnoside (Afzelin) in Normotensive and Hypertensive Rats.
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-05-26 , DOI: 10.1021/acs.jnatprod.0c00274 Camile Cecconi Cechinel-Zanchett 1 , Luísa Nathália Bolda Mariano 1 , Thaise Boeing 1 , Juliana de Carvalho da Costa 2 , Luísa Mota Da Silva 1 , Jairo K Bastos 2 , Valdir Cechinel-Filho 1 , Priscila de Souza 1
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-05-26 , DOI: 10.1021/acs.jnatprod.0c00274 Camile Cecconi Cechinel-Zanchett 1 , Luísa Nathália Bolda Mariano 1 , Thaise Boeing 1 , Juliana de Carvalho da Costa 2 , Luísa Mota Da Silva 1 , Jairo K Bastos 2 , Valdir Cechinel-Filho 1 , Priscila de Souza 1
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Our previous study showed that kaempferitrin, the main flavonoid from Bauhinia forficata Link leaves, induces diuresis and saluresis when orally given to rats. Since afzelin (AFZ) and kaempferol (KFL) are active compounds from the biometabolism of kaempferitrin, the diuretic and renal protective properties of these two compounds were evaluated. While the acute treatment with AFZ evoked a diuretic action associated with an increase in Cl– excretion and a Ca2+-sparing effect, KFL did not present any activity. The pretreatment with a muscarinic receptor blocker or with an inhibitor of the cyclooxygenase fully avoided AFZ-induced diuresis. AFZ also induced a prolonged (7-day treatment) diuretic effect in normotensive (NTR) and hypertensive rats (SHR), with an increase of urinary Na+ and Cl– excretion, while it decreased the elimination of Ca2+. AFZ was able to decrease ROS and nitrite generation on kidney homogenates in comparison with the SHR group treated with the vehicle, as well as mitigated the changes in the renal corpuscle region (glomerulus and Bowman’s capsule). Moreover, AFZ significantly reduced calcium oxalate crystal formation in urine, with inhibition rates of 41% for the NTR and 92% for the SHR group. Taken together, this study shows that AFZ exerts acute and prolonged diuretic effects plus protective renal properties.
中文翻译：
Kaempferol 3-O-Alpha-l-rhamnoside (Afzelin) 对正常血压和高血压大鼠的利尿和肾脏保护作用。
我们之前的研究表明山柰素是紫荆叶中的主要黄酮类化合物，当给大鼠口服时，可诱导利尿和排尿。由于afzelin (AFZ) 和山奈酚(KFL) 是来自山奈酚生物代谢的活性化合物，因此评估了这两种化合物的利尿和肾脏保护特性。虽然 AFZ 的急性治疗引起了与 Cl -排泄和 Ca 2+增加相关的利尿作用-保留效应，KFL 没有表现出任何活性。用毒蕈碱受体阻滞剂或环加氧酶抑制剂预处理完全避免了AFZ诱导的利尿。AFZ 还在正常血压 (NTR) 和高血压大鼠 (SHR) 中诱导延长（7 天治疗）利尿作用，增加尿 Na +和 Cl -排泄，同时减少 Ca 2+的消除. 与用载体处理的 SHR 组相比，AFZ 能够减少肾匀浆中的 ROS 和亚硝酸盐生成，并减轻肾小体区域（肾小球和鲍曼囊）的变化。此外，AFZ 显着减少尿液中草酸钙晶体的形成，NTR 组抑制率为 41%，SHR 组抑制率为 92%。总之，这项研究表明 AFZ 具有急性和长期利尿作用以及保护性肾脏特性。
更新日期：2020-06-26
中文翻译：
Kaempferol 3-O-Alpha-l-rhamnoside (Afzelin) 对正常血压和高血压大鼠的利尿和肾脏保护作用。
我们之前的研究表明山柰素是紫荆叶中的主要黄酮类化合物，当给大鼠口服时，可诱导利尿和排尿。由于afzelin (AFZ) 和山奈酚(KFL) 是来自山奈酚生物代谢的活性化合物，因此评估了这两种化合物的利尿和肾脏保护特性。虽然 AFZ 的急性治疗引起了与 Cl -排泄和 Ca 2+增加相关的利尿作用-保留效应，KFL 没有表现出任何活性。用毒蕈碱受体阻滞剂或环加氧酶抑制剂预处理完全避免了AFZ诱导的利尿。AFZ 还在正常血压 (NTR) 和高血压大鼠 (SHR) 中诱导延长（7 天治疗）利尿作用，增加尿 Na +和 Cl -排泄，同时减少 Ca 2+的消除. 与用载体处理的 SHR 组相比，AFZ 能够减少肾匀浆中的 ROS 和亚硝酸盐生成，并减轻肾小体区域（肾小球和鲍曼囊）的变化。此外，AFZ 显着减少尿液中草酸钙晶体的形成，NTR 组抑制率为 41%，SHR 组抑制率为 92%。总之，这项研究表明 AFZ 具有急性和长期利尿作用以及保护性肾脏特性。
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