The current study was carried out to compare the antileishmanial potential of the characterized chemically and plant mediated (Trigonella foenum-graecum) FeO-NPs (FeO-NPs) against L.tropica KWH23. The promastigotes and amastigotes mortality, ROS generation, and biocompatibility assessment were estimated in time-dose dependent manner by PDT. LED exposed bio-inspired FeO-NPs after 72 hours, express significant suppressive effects by exhibiting 50% inhibitory concentration (IC50) for promastigotes and amastigotes, respectively. Leishmanial DNA damage and membrane integrity caused by FeO-NPs were owe to high production of reactive oxygen species (ROS). By applying different ROS scavengers (mannitol and sodium azide) hydroxyl radical and singlet oxygen were found as main moieties for cell death by giving quantum yield 0.15 and 0.28 through chemically and green synthesized FeO-NPs, respectively. The light exposed green synthesized nanoparticles were found biocompatible on human red blood cells (RBCs), by exhibiting LD50 value 2659μg/mL. From these findings, it can be concluded that plant mediated FeO-NPs can be used as promising antiprotozoal agent.

中文翻译：

---

化学和生物合成的氧化铁纳米颗粒对热带利什曼原虫的比较分析

进行了当前的研究，以比较表征的化学和植物介导的（Trigonella foenum-graecum）FeO-NPs（FeO-NPs）与L.tropica KWH23的抗菌作用。通过PDT以时间剂量依赖性的方式估计了前鞭毛体和变形虫的死亡率，ROS的产生以及生物相容性评估。受LED照射的受生物启发的FeO-NPs在72小时后表现出显着的抑制作用，分别对前鞭毛体和变形虫表现出50％的抑制浓度（IC50）。FeO-NP引起的利什曼病毒DNA损伤和膜完整性归因于活性氧（ROS）的大量产生。通过应用不同的ROS清除剂（甘露醇和叠氮化钠），通过给出量子产率0.15和0，发现羟基自由基和单线态氧是细胞死亡的主要部分。通过化学和绿色合成的FeO-NPs分别获得28种。通过显示LD50值为2659μg/ mL，发现曝光的绿色合成纳米颗粒在人红细胞（RBC）上具有生物相容性。从这些发现，可以得出结论，植物介导的FeO-NP可以用作有希望的抗原生动物剂。