The aim of the study was to investigate if there are any differences in effects of extracts of commercially (GC) and alternatively (wheat straw) (GA) cultivated Ganoderma lucidum basidiocarps on properties of peritoneal macrophages (PM) and monocyte-derived dendritic cells (MoDCs). GA extract stronger stimulated the metabolic and phagocytic activity of PMs, their adhesion capability and ability to produce ROS and NO compared to GC. Both tested extracts significantly increased allostimulatory and Th1 polarization capacity of simultaneous TLR3 and TLR7-activated MoDCs, but GA extract was more effective. The GA extract increased the production of ROS and NO by TLR4 stimulated PMs and up-regulated the production of certain cytokines as well as allostimulatory and Th1 polarization capacity of MoDCs. The GA extract could be a potent immunostimulatory agent for activation of MoDCs with the simultaneous engagement of TLRs that seems to be a promising strategy for preparation of DC-based anti-tumor vaccines.

中文翻译：

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在其他基质上培养的灵芝基础果皮提取物的免疫调节作用

这项研究的目的是研究商业（GC）和（小麦秸秆）（GA）栽培的灵芝基础果皮提取物对腹膜巨噬细胞（PM）和源自单核细胞的树突状细胞的特性是否有任何差异（ MoDC）。与GC相比，GA提取物更能刺激PM的代谢和吞噬活性，它们的粘附能力以及产生ROS和NO的能力。两种测试提取物均显着提高了同时TLR3和TLR7激活的MoDC的同素刺激和Th1极化能力，但GA提取物更有效。GA提取物通过TLR4刺激的PMs增加了ROS和NO的生成，并上调了某些细胞因子的生成以及MoDC的同素刺激和Th1极化能力。