Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are amongst the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organisation and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, Core Fermentation (CoFe) granules; they appear to represent translation factories allowing high-level co-ordinated enzyme synthesis for a critical metabolic pathway.

中文翻译：

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核心发酵（CoFe）颗粒专注于协调的糖酵解mRNA定位和翻译，以促进葡萄糖发酵

糖酵解是整个生物学中葡萄糖分解代谢的基本代谢途径，糖酵解酶是细胞中最丰富的蛋白质之一。它们在这种水平上的表达提出了特别的挑战。在这里，我们证明了糖酵解的mRNA位于酵母和人类细胞中的颗粒。酵母中详细的活细胞和smFISH研究表明，mRNA在颗粒中活跃地翻译，这种翻译对于定位至关重要。此外，这种布置可能促进糖酵解途径的更高水平的组织和控制。实际上，细胞所需的发酵程度与mRNA定位于颗粒的程度内在相关。在此基础上，我们将这些颗粒称为核心发酵（CoFe）颗粒。