Scattering is a huge challenge for microscopic imaging. Indeed, it is difficult to observe target chemicals in scattering media by means of the current Gaussian beam-based stimulated Raman scattering (SRS) microscopy, since the tight focus of the Gaussian beam is destroyed after propagating through a certain distance. Bessel beams, featuring self-reconstructing property, may bring a solution to this problem. By combining Bessel beams with SRS microscopy, we can probe the SRS signal from a scattering medium. In this paper, using the beam propagation method, we first simulate the propagation of the Bessel beam as well as the generation and self-reconstruction of SRS signals. By adding glass beads along the beam propagation path in order to simulate scattering, the propagation of the Bessel beams and the generation of the SRS signals will change. Then, we design a series of simulations to investigate the influence of the size, position, number, and distribution of the added glass beads on the generation of the SRS signals. A preliminary experiment is also carried out to confirm the simulation predictions. Results demonstrate that the SRS signals can be generated or be recovered at a certain depth in scattering media, and that such signals are greatly affected by the parameters of the scatters.

中文翻译：

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使用自重构贝塞尔光束在散射介质中生成受激拉曼散射信号

散射是显微成像的巨大挑战。事实上，目前的基于高斯光束的受激拉曼散射 (SRS) 显微镜很难观察散射介质中的目标化学物质，因为高斯光束的紧密焦点在传播一定距离后会被破坏。具有自重构特性的贝塞尔梁可能会为这个问题带来解决方案。通过将贝塞尔光束与 SRS 显微镜相结合，我们可以探测来自散射介质的 SRS 信号。本文首先采用波束传播的方法模拟贝塞尔波束的传播以及SRS信号的产生和自重构。通过沿光束传播路径添加玻璃珠以模拟散射，贝塞尔光束的传播和 SRS 信号的生成将发生变化。然后，我们设计了一系列模拟来研究添加玻璃珠的大小、位置、数量和分布对 SRS 信号生成的影响。还进行了初步实验以确认模拟预测。结果表明，在散射介质中一定深度可以产生或恢复SRS信号，并且这种信号受散射参数的影响很大。