Abstract

Introduction

Anxiety is an important factor for self-administered alcohol as a tool to reduce its symptoms. However, alcohol is capable to disrupt sleep-wake patterns in subjects with medium- to high-alcohol consumption. We have selectively bred two sublines from Sprague-Dawley rats that differ on its yawning frequency. High-yawning (HY) rats have a mean of 20 yawns/h, whereas the Low-yawning (LY) rats have only 2 yawns/hour. LY rats also showed anxious responses when evaluated on standardized tests. The aim of this study was to assess the changes on sleep-wake patterns after chronic alcohol consumption.

Methods

We used 8 males from HY and LY sublines at 3 months of age, they lived in acrylic cages with water and food pellets available ad libitum under a 12:12 light-dark cycle (lights on at 0700) and temperature of 21±1 °C. All subjects were implanted to record EEG, EMG and EOG to characterize sleep-wake phases. A baseline sleep-wake recording was obtained for 24 h. A solution of 9.6% alcohol was administered as a single source of hydration for seven days and then a second sleep-wake recording was obtained. After that period, an additional bottle containing purified water was available. Position of the bottles was randomly changed daily. Water and alcohol consumption were measured daily for a period of 3 weeks and then a third sleep-wake recording was obtained.

Results

LY rats consumed more alcohol than HY rats (P<0.05), and they had an increase of bouts and duration of slow wave sleep and REM sleep on their active phase after alcohol administration (P<0.05).

Conclusion

LY rats display an anxious behavior and therefore consumed more alcohol compared to HY rats, and only LY rats were susceptible to alcohol effects on sleep on their active phase.

Support

Partially supported by CONACYT grants 243333 and 243247 to CC and JRE, respectively. Grants from VIEP-BUAP 2018 and CA in Neuroendocrinología BUAP-CA-288.

中文翻译：

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0249饮酒对焦虑模型大鼠睡眠-觉醒模式的影响

摘要

介绍

焦虑是自我管理酒精减轻症状的重要因素。然而，在中度至高度饮酒的人群中，酒精能够破坏睡眠-觉醒模式。我们从Sprague-Dawley大鼠中选择了两个亚种，它们的打哈欠频率不同。打哈欠（HY）的老鼠平均每小时打20个哈欠，而打低哈欠（LY）的老鼠每小时只打2个哈欠。当在标准化测试中评估时，LY大鼠还表现出焦虑反应。这项研究的目的是评估长期饮酒后睡眠觉醒模式的变化。

方法

我们使用了8个来自HY和LY子系的雄性3个月大的雄性，它们生活在丙烯酸笼中，水和食物颗粒可自由采摘，在12:12的明暗周期（0700点亮）下，温度为21±1° C。植入所有受试者以记录EEG，EMG和EOG以表征睡眠-觉醒阶段。获得24小时的基线睡眠-觉醒记录。将9.6％的酒精溶液作为单一水合来源给药7天，然后获得第二次睡眠唤醒记录。在此期间之后，可以再使用一个装有纯净水的瓶子。每天随机改变瓶子的位置。每天测量水和酒精的消耗量，持续3周，然后进行第三次睡眠-觉醒记录。

结果

LY大鼠饮酒比HY大鼠多（P <0.05），饮酒后活动期的发作次数增加，慢波睡眠和REM睡眠持续时间增加（P <0.05）。

结论

LY大鼠表现出焦虑行为，因此与HY大鼠相比消耗更多的酒精，只有LY大鼠在活动期对睡眠的酒精敏感。

支持

由CONACYT部分支持，分别向CC和JRE授予243333和243247。VIEP-BUAP 2018和CA在NeuroendocrinologíaBUAP-CA-288中的拨款。