Abstract

Candida albicans and Candida glabrata are frequently coisolated from the oral cavity in immunosuppressive or immunocompromised individuals. Their relationship is usually defined as competition as C. glabrata can inhibit growth of C. albicans in cohabitation. In this study, eight C. albicans isolates as well as two C. glabrata strains were used to investigate the effects of culture medium (Roswell Park Memorial Institute [RPMI]-1640, YPD, YND), incubation time (24 h, 48 h, 72 h, 96 h), initial inoculum (C. glabrata: C. albicans = 2:1, 1:1, 1:2), and medium state (static and dynamic states) on viable cell enumeration and relative abundance in both Candida SB and MB. The results showed that in most cases, C. glabrata and C. albicans SB and MB flourished in RPMI-1640 at 24 h under dynamic state compared with other conditions. Except YPD medium, there were high proportions of preponderance of C. albicans over C. glabrata in MB compared with SB. High initial inoculum promoted corresponding Candida number in both SB and MB and its abundance in MB relative to SB. This study revealed an impact of several environmental conditions on the formation of C. albicans and C. glabrata SB and MB and their abundance in MB in comparison with SB, deepening our understanding of both Candida interaction and their resistance mechanism in MB.

Lay Summary

This study described the effects of diverse experimental conditions on the numbers of Candida albicans and Candida glabrata single biofilms and mixed biofilms and their abundance.

中文翻译：

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不同条件下白色念珠菌和光滑念珠菌混合生物膜的丰度相互作用

摘要

白色念珠菌和光滑念珠菌经常在免疫抑制或免疫功能低下的个体中从口腔中分离出来。它们的关系通常被定义为竞争，因为C. glabrata可以抑制C. albicans在同居中的生长。在这项研究中，使用八种白色念珠菌分离株和两种光滑念珠菌菌株来研究培养基（罗斯威尔公园纪念研究所 [RPMI]-1640、YPD、YND）、孵育时间（24 小时、48 小时）的影响。 , 72 h, 96 h), 初始接种物 ( C. glabrata: C. albicans  = 2:1, 1:1, 1:2) 和中等状态（静态和动态）的活细胞计数和两者的相对丰度念珠菌SB和MB。结果表明，在大多数情况下，与其他条件相比，在动态状态下，光滑念珠菌和白色念珠菌SB和MB在RPMI-1640中24 h的生长旺盛。除 YPD 培养基外，与 SB 相比，MB中白色念珠菌优于光滑念珠菌的比例较高。高初始接种量促进了 SB 和 MB 中相应的念珠菌数量及其在 MB 中相对于 SB 的丰度。本研究揭示了几种环境条件对白色念珠菌和光滑念珠菌SB 和 MB 形成的影响，以及与 SB 相比它们在 MB 中的丰度，加深了我们对念珠菌的理解。MB中的相互作用及其耐药机制。

总结

本研究描述了不同实验条件对白色念珠菌和光滑念珠菌单一生物膜和混合生物膜数量及其丰度的影响。