Abstract

The complete mitogenome of Saccharum spp. hybrid FN15 was successfully sequenced. It contains two distinct circular chromosomes, Chromosome 1 and Chromosome 2. The former is 301,533 bp in length with the GC content of 43.90%, and 7.12% of genome (21,468 nucleotides) are coding DNA while 92.88% of genome (280,065 nucleotides) are intergenic region. The latter is 144,744 bp in length with the GC content of 43.57%, and 8.20% of genome (11,865 nucleotides) are coding DNA and 91.80% of genome (132,879 nucleotides) are intergenic region. Besides, Chromosome 1 contains 22 protein-coding genes (four atp genes, three ccm genes, three cox genes, one mat gene, one mtt gene, six nad genes and four rps genes), and 21 non-coding genes (15 tRNA and six rRNAs), whereas in Chromosome 2, there are 13 protein-coding genes (two atp genes, one ccm gene, one cob gene, one cox gene, one rpl gene, four nad genes and three rps genes) and five tRNA genes. Maximum Likelihood phylogenetic analysis demonstrated that FN15 is close with S. spp. hybrid ROC22, S. officinarum Khon Kaen 3 and S. bicolor species. This complete mitochondrial genome will provide essential DNA molecular data for further phylogenetic and evolutionary analysis for Saccharum.

摘要

的完整线粒体基因甘蔗属。杂交FN15已成功测序。它包含两个不同的环状染色体，染色体1和染色体2。前者的长度为301,533 bp，GC含量为43.90％，基因组的7.12％（21,468个核苷酸）编码DNA，而基因组的92.88％（280,065个核苷酸）为DNA。基因间区域。后者的长度为144,744 bp，GC含量为43.57％，基因组的8.20％（11,865个核苷酸）编码DNA，基因组的91.80％（132,879个核苷酸）。此外，染色体1包含22个蛋白质编码基因（四个atp基因，三个ccm基因，三个cox基因，一个mat基因，一个mtt基因，六个nad基因和4个rps基因）和21个非编码基因（15个tRNA和6个rRNA），而在染色体2中，有13个蛋白质编码基因（两个atp基因，一个ccm基因，一个cob基因，一个cox基因，一个rpl基因，四个nad基因和三个rps基因）和五个tRNA基因。最大似然系统发育分析表明，FN15靠近与S.属。混合ROC22，S.良姜孔敬府3和S.双色种类。这个完整的线粒体基因组将提供重要的DNA分子数据，用于进一步对蔗糖进行系统发育和进化分析。