Abstract

Betula chichibuensis is a critically endangered limestone birch confined to the Chichibu and Kitakami mountains in central and northeastern Japan, respectively. In this study, we assembled and characterized the complete chloroplast genome of B. chichibuensis. The whole chloroplast genome was 160,791 bp in length, consisting of a large single-copy (LSC) region of 89,504 bp and a small single-copy (SSC) region of 19,175 bp, separated by a pair of inverted repeat (IR) regions of 26,056 bp. It contained 133 genes, including 88 protein-coding genes (80 PCG types), 37 tRNA genes (30 tRNA types), and eight rRNA genes (four rRNA types). The overall GC content of the chloroplast genome was 36.01%. Phylogenetic analysis resolved B. chichibuensis as sister to the clade containing B. pendula.

摘要

桦chichibuensis是局限在日本中部和东北部的秩父和北上山脉极度濒危石灰石桦木，分别。在这项研究中，我们组装并表征了秩父细菌的完整叶绿体基因组。整个叶绿体基因组的长度为160,791 bp，由89,504 bp的大单拷贝（LSC）区和19,175 bp的小单拷贝（SSC）区组成，并由一对反向重复序列（IR）隔开26,056 bp。它包含133个基因，包括88个蛋白质编码基因（80种PCG类型），37个tRNA基因（30个tRNA类型）和8个rRNA基因（四种rRNA类型）。叶绿体基因组的总GC含量为36.01％。系统发育分析解析秩父肠杆菌为含有该进化枝的姐妹。B.摆。