Abstract

The objective of this work is to develop cost-effective, reliable, and large-scale production of metallic nanoparticles (NPs) by adopting green chemistry principles for industrial applications. In that view, we have studied the phytochemical reducibility of silver nitrate by making use of Calotropis Procera (Ait.) R. Br root extract, and based on its medicinal properties, an attempt was made to evaluate the therapeutic potentials of silver (Ag) NPs containing this plant extract towards the clinical strains of bacteria. The optimization studies on reducing the potentials were done considering the concentration, pH, temperature, and reaction period of both the extract and the metal precursor. The nanoarchitecture elements were interpreted using visual, spectroscopic, and microscopic analyses cohorting the antibacterial potentials towards the clinically significant strains. The antimicrobial activity exercised by these Ag NPs towards 10 different strains of medically important bacteria at a given concentration was proved to be significant. The antimicrobial potential was further validated quantitatively, and the MIC/MBC concentration values were determined. Finally, the cytotoxicity of Ag NPs when tested against the HEPK cell line indicated that the metal-phytochemical moiety exhibited the maximum therapeutic efficacy and thereby paving the way for the development of disruptive technologies in the field of nanomedicine.

摘要

这项工作的目的是通过采用绿色化学原理进行工业应用，以开发经济高效，可靠且大规模的金属纳米颗粒（NP）。有鉴于此，我们已经通过使用Calotropis Procera（Ait。）R. Br根提取物研究了硝酸银的植物化学还原性，并基于其药用特性，尝试评估银（Ag）的治疗潜力。含有这种植物的NPs可以提取临床细菌菌株。考虑到萃取液和金属前体的浓度，pH，温度和反应时间，进行了降低电位的优化研究。纳米结构元素通过视觉，光谱，和显微镜分析，对具有临床意义的菌株进行抗菌潜能分析。这些银纳米颗粒在给定浓度下对10种不同的医学上重要细菌的抗菌活性被证明是有效的。进一步对抗菌潜力进行了定量验证，并确定了MIC / MBC浓度值。最后，当针对HEPK细胞系进行测试时，Ag NPs的细胞毒性表明金属植物化学部分表现出最大的治疗功效，从而为纳米医学领域破坏性技术的发展铺平了道路。进一步对抗菌潜力进行了定量验证，并确定了MIC / MBC浓度值。最后，当针对HEPK细胞系进行测试时，Ag NPs的细胞毒性表明金属植物化学部分表现出最大的治疗功效，从而为纳米医学领域破坏性技术的发展铺平了道路。进一步对抗菌潜力进行了定量验证，并确定了MIC / MBC浓度值。最后，当针对HEPK细胞系进行测试时，Ag NPs的细胞毒性表明金属植物化学部分表现出最大的治疗功效，从而为纳米医学领域破坏性技术的发展铺平了道路。