The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria.,Antibiotics

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The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria.
Antibiotics ( IF 4.3 ) Pub Date : 2020-05-27 , DOI: 10.3390/antibiotics9060286
Ali F Saleem ₁ , Ahreen Allana ₁ , Lauren Hale ₂ , Alondra Diaz ₃ , Raul Salinas ₂ , Cristina Salinas ₂ , Shahida M Qureshi ₁ , Aneeta Hotwani ₁ , Najeeb Rahman ₁ , Asia Khan ₁ , Anita K Zaidi ₁ , Patrick C Seed _{3,

4,

5} , Mehreen Arshad _{2,

3,

4,

5}

Affiliation

The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae among healthy infants born in Pakistan, a setting with high incidence of MDR-E infections. Stool samples were collected from 104 healthy infants between the ages of 5 and 7 months. Enterobacteriaceae isolates were screened for resistance against several antimicrobial classes. Presence of ESBL and carbapenemase genes was determined using multiplex PCR. Sequence types were assigned to individual strains by multi-locus sequence typing. Phylogenetic analysis of Escherichia coli was done using the triplex PCR method. Forty-three percent of the infants were positive for ESBL-producing Enterobacteriaceae, the majority of which were E. coli. We identified several different ESBL E. coli sequence types most of which belonged to the phylogenetic group B2 (23%) or D (73%). The widespread colonization of infants in a developing country with ESBL-producing Enterobacteriaceae is concerning. The multiple sequence types and reported non-human sources support that multiple non-epidemic MDR lineages are circulating in Pakistan with healthy infants as a common reservoir.

更新日期：2020-05-27

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