Induction of γ-aminobutyric acid plays a positive role to Arabidopsis resistance against Pseudomonas syringae.,Journal of Integrative Plant Biology

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Induction of γ-aminobutyric acid plays a positive role to Arabidopsis resistance against Pseudomonas syringae.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-05-26 , DOI: 10.1111/jipb.12974
Xiangxiong Deng ₁ , Xuwen Xu ₁ , Yu Liu ₁ , Yan Zhang ₁ , Liuyi Yang ₁ , Shuqun Zhang ₂ , Juan Xu ₁

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Gamma‐aminobutyric acid (GABA) is an important metabolite which functions in plant growth, development, and stress responses. However, its role in plant defense and how it is regulated are largely unknown. Here, we report a detailed analysis of GABA induction during the resistance response to Pseudomonas syringae in Arabidopsis thaliana. While searching for the mechanism underlying the pathogen‐responsive mitogen‐activated protein kinase (MPK)3/MPK6 signaling cascade in plant immunity, we found that activation of MPK3/MPK6 greatly induced GABA biosynthesis, which is dependent on the glutamate decarboxylase genes GAD1 and GAD4. Inoculation with Pseudomonas syringae pv tomato DC3000 (Pst) and Pst‐avrRpt2 expressing the avrRpt2 effector gene induced GAD1 and GAD4 gene expression and increased the levels of GABA. Genetic evidence revealed that GAD1, GAD2, and GAD4 play important roles in both GABA biosynthesis and plant resistance in response to Pst‐avrRpt2 infection. The gad1/2/4 triple and gad1/2/4/5 quadruple mutants, in which the GABA levels were extremely low, were more susceptible to both Pst and Pst‐avrRpt2. Functional loss of MPK3/MPK6, or their upstream MKK4/MKK5, or their downstream substrate WRKY33 suppressed the induction of GAD1 and GAD4 expression after Pst‐avrRpt2 treatment. Our findings shed light on both the regulation and role of GABA in the plant immunity to a bacterial pathogen.

中文翻译：

γ-氨基丁酸的诱导对于拟南芥对丁香假单胞菌的抗性具有积极作用。

γ-氨基丁酸（GABA）是一种重要的代谢产物，在植物的生长，发育和胁迫反应中起作用。但是，其在植物防御中的作用及其调控方式尚不清楚。在这里，我们的性反应过程中报告GABA诱导的详细分析丁香假单胞菌在拟南芥。在寻找病原体反应性促分裂原激活蛋白激酶（MPK）3 / MPK6信号级联在植物免疫中的基础机制时，我们发现MPK3 / MPK6的激活极大地诱导了GABA的生物合成，这取决于谷氨酸脱羧酶基因GAD1和GAD4。接种丁香假单胞菌PV番茄DC3000（PST）和PST-avrRpt2表达avrRpt2诱导效应基因GAD1和GAD4基因表达和增加的GABA的水平。遗传证据表明，GAD1，GAD2和GAD4在GABA生物合成和植物抗Pst-avrRpt2感染的抗性中均起重要作用。所述GAD1 / 2/4三重和GAD1 / 2/4/5四突变体，其中所述GABA水平非常低，分别为这两个更敏感的Pst和PST-avrRpt2。在Pst- avrRpt2处理后，MPK3 / MPK6或其上游MKK4 / MKK5或其下游底物WRKY33的功能丧失抑制了GAD1和GAD4表达的诱导。我们的发现揭示了GABA在植物对细菌病原体免疫中的调节作用和作用。

更新日期：2020-05-26

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