Haplotype‐based breeding, a recent promising breeding approach to develop tailor‐made crop varieties, deals with identification of superior haplotypes and their deployment in breeding programmes. In this context, whole genome re‐sequencing data of 292 genotypes from pigeonpea reference set were mined to identify the superior haplotypes for 10 drought‐responsive candidate genes. A total of 83, 132 and 60 haplotypes were identified in breeding lines, landraces and wild species, respectively. Candidate gene‐based association analysis of these 10 genes on a subset of 137 accessions of the pigeonpea reference set revealed 23 strong marker‐trait associations (MTAs) in five genes influencing seven drought‐responsive component traits. Haplo‐pheno analysis for the strongly associated genes resulted in the identification of most promising haplotypes for three genes regulating five component drought traits. The haplotype C. cajan_23080‐H2 for plant weight (PW), fresh weight (FW) and turgid weight (TW), the haplotype C. cajan_30211‐H6 for PW, FW, TW and dry weight (DW), the haplotype C. cajan_26230‐H11 for FW and DW and the haplotype C. cajan_26230‐H5 for relative water content (RWC) were identified as superior haplotypes under drought stress condition. Furthermore, 17 accessions containing superior haplotypes for three drought‐responsive genes were identified. The identified superior haplotypes and the accessions carrying these superior haplotypes will be very useful for deploying haplotype‐based breeding to develop next‐generation tailor‐made better drought‐responsive pigeonpea cultivars.

中文翻译：

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木豆（Cajanus cajan L.）基于单倍型的耐旱育种的优良单倍型。

基于单体型的育种是开发有针对性的农作物品种的最新有前途的育种方法，用于鉴定优良单体型及其在育种计划中的部署。在这种情况下，对来自木豆参考集的292个基因型的全基因组重测序数据进行了挖掘，以确定10个干旱响应候选基因的优良单倍型。在育种系，地方品种和野生物种中分别鉴定出总共83、132和60个单倍型。对木豆参考集的137个种质的这10个基因进行基于候选基因的关联分析，揭示了影响7个干旱响应性状的5个基因中的23个强标记-性状关联（MTA）。单倍型对强相关基因的分析导致鉴定出调节五个成分干旱性状的三个基因的最有前途的单倍型。单倍型C. cajan_23080 -H2植物重量（PW），鲜重（FW）和肿胀重量（TW），单倍型C. cajan_30211- H6为PW，FW，TW和干重（DW），单倍型C.适用于FW和DW的cajan_26230- H11和单倍型C.cajan_26230‐H5相对水分含量（RWC）被确定为干旱胁迫条件下的优良单倍型。此外，还鉴定出17个包含三个干旱响应基因的优良单倍型的种质。鉴定出的优良单倍型和携带这些优良单倍型的种质对于部署基于单倍型的育种来开发下一代定制的，更好的干旱响应性木豆品种将非常有用。