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Purification of cell-derived Japanese encephalitis virus by dual-mode chromatography
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-05-27 , DOI: 10.1002/bab.1960 Fuliang Zhang 1, 2 , Jun Luo 3 , Man Teng 3 , Guangxu Xing 3 , Junqing Guo 3 , Yihua Zhang 1
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-05-27 , DOI: 10.1002/bab.1960 Fuliang Zhang 1, 2 , Jun Luo 3 , Man Teng 3 , Guangxu Xing 3 , Junqing Guo 3 , Yihua Zhang 1
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Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and CaptoTM Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large-scale production of high-purity JEV.
中文翻译：
双模式层析法纯化细胞源性乙型脑炎病毒
包膜病毒的纯化是一项挑战，因为必须保持病毒感染性以保持免疫原性。传统的病毒纯化过程耗时、费力且难以扩大规模。在这里，通过使用差速离心、超滤、Sepharose 4 快速流动凝胶色谱和 Capto TM Core 700 色谱开发了一种用于纯化日本脑炎病毒 (JEV) 的快速、简单和可扩展的实验室程序。整个过程回收了61.64%的原始病毒，纯化后的病毒颗粒保持了良好的活性和免疫原性。所描述的净化过程在高纯度 JEV 的大规模生产中具有潜在的应用。
更新日期：2020-05-27
中文翻译：
双模式层析法纯化细胞源性乙型脑炎病毒
包膜病毒的纯化是一项挑战，因为必须保持病毒感染性以保持免疫原性。传统的病毒纯化过程耗时、费力且难以扩大规模。在这里，通过使用差速离心、超滤、Sepharose 4 快速流动凝胶色谱和 Capto TM Core 700 色谱开发了一种用于纯化日本脑炎病毒 (JEV) 的快速、简单和可扩展的实验室程序。整个过程回收了61.64%的原始病毒，纯化后的病毒颗粒保持了良好的活性和免疫原性。所描述的净化过程在高纯度 JEV 的大规模生产中具有潜在的应用。
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