To enrich the properties of chitosan (CS) and improve its applicability, we prepared carboxymethyl-chitosan (CMCS) by carboxymethylation and developed a layer-by-layer assembly (LBL) by the ultrasonic nebulization-assisted technique. The degree of carboxymethylation was 42.20% and the structure of CMCS was characterized using Fourier transform infrared, nuclear magnetic resonance spectroscopy. The ultrasonic nebulization-assisted LBL (UNLBL) edible coating was developed based on an inner negatively CMCS charged layer and an external positively charged CS layer. As compared to conventional LBL (CLBL) assembly, the greater thickness, stronger mechanical properties and lower water vapor permeability were detected in UNLBL assembly. Through an untargeted metabolomic analysis and real-time quantitative PCR, it was evident that ultrasonic nebulization-assisted LBL assembly is a promising alternative for promoting phenylpropanoid metabolism in strawberries.

为了丰富壳聚糖（CS）的性能并提高其适用性，我们通过羧甲基化制备了羧甲基壳聚糖（CMCS），并通过超声雾化辅助技术开发了逐层组装（LBL）。羧甲基化度为42.20％，使用傅里叶变换红外核磁共振波谱表征了CMCS的结构。超声波雾化辅助LBL（UNLBL）食用涂料是基于内部带负电荷的CMCS和外部带正电荷的CS层而开发的。与常规LBL（CLBL）组件相比，在UNLBL组件中检测到更大的厚度，更强的机械性能和更低的水蒸气渗透性。通过非目标代谢组学分析和实时定量PCR，