Low Fe availability affects plant production mainly by impairing the photosynthetic pathway, since Fe plays an essential role in chlorophyll synthesis as well as in the photosynthetic electron transport chain. Under these conditions, plant cells require the activation of protective mechanisms to prevent photo-inhibition. Among these mechanisms, photorespiration (PR) has been relatively little investigated in Fe-deficient plants.

The aim of this work was to investigate the effect of Fe deficiency on photorespiration by performing in vivo analysis in leaves as well as biochemical characterization of some PR-related enzyme activities in a peroxisome-purified fraction from cucumber leaves. Modelling of light response curves at both 21 and 2% pO₂ revealed a slowing down of PR under Fe deficiency. The activity of some PR-involving enzymes as well as the contents of glycine and serine were affected under Fe deficiency. Furthermore, nitrate reductase, the glutamine synthetase-glutamate synthase (GS-GOGAT) cycle and hydroxypyruvate dehydrogenase isoform activities were differentially altered under Fe deficiency. The dataset indicates that, in Fe-deficient cucumber leaves, the modulation of PR involves the induction of some PR-related pathways, such as the photorespiratory N recycling and cytosolic photorespiratory bypass processes.

由于铁在叶绿素合成以及光合电子传输链中起着至关重要的作用，所以铁的低利用率主要通过损害光合作用途径来影响植物的生产。在这些条件下，植物细胞需要激活保护机制来防止光抑制。在这些机制中，在缺铁植物中光呼吸（PR）的研究相对较少。

这项工作的目的是通过在叶片中进行体内分析以及在过氧化物酶体纯化的黄瓜叶片中某些与PR相关的酶活性的生化特征中研究铁缺乏对光呼吸的影响。pO ₂为21％和2％时的光响应曲线建模揭示了铁缺乏引起的PR减慢。铁缺乏会影响某些参与PR的酶的活性以及甘氨酸和丝氨酸的含量。此外，在铁缺乏的情况下，硝酸还原酶，谷氨酰胺合成酶-谷氨酸合酶（GS-GOGAT）循环和羟基丙酮酸脱氢酶同工型活性也有差异。数据集表明，在缺铁的黄瓜叶片中，PR的调节涉及一些与PR相关的途径的诱导，例如光呼吸氮循环和胞质光呼吸旁路过程。