The present study was developed to characterize, at the species level, 34 strains of Aeromonas spp., previously isolated from stressed tambaqui fish (Colossoma macropomum), to elucidate virulence factors, as well as their antibiotic resistance profile. Amplification of the gyrB gene identified the strains as A. hydrophila, A. dhakensis, A. caviae, A. veronii and A. jandaei. Bacterial virulence was evaluated by enzymatic assays for phenotypical production of hemolysins, proteases and lipases followed by the search for genes codifying the enzymes β-hemolysin, serine protease and lipase. Phenotypical production of virulence factors was diversified and proteolytic activity demonstrated to be a common expression among the strains. On the other hand, the lip gene encoding extracellular lipase was the most expressed. Furthermore, A. hydrophila was the most prevalent species isolated from tambaqui in our work.

进行本研究的目的是在物种水平上表征34种先前从重压坦巴基鱼（Colossoma macropomum）中分离的气单胞菌菌株，以阐明毒力因子及其抗生素抗性。所述的放大的gyrB基因鉴定的菌株为嗜水，甲。dhakensis，A.豚鼠气，A.维罗纳和A. jandaei。通过酶法测定溶血素，蛋白酶和脂肪酶的表型产生的细菌毒力，然后搜索将β-溶血素，丝氨酸蛋白酶和脂肪酶的基因编码。毒力表型的产生多样化，蛋白水解活性被证明是菌株之间的共同表达。另一方面，编码细胞外脂肪酶的嘴唇基因表达最多。此外，嗜水气单是从坦巴基我们工作中分离出的最常见的种类。