Gas fermentation for the production of building block molecules and biofuels is lately gaining attention as a means to eliminate the greenhouse gases emissions. Especially CO₂ capture and recycling are in focus. Thus, the biological coupling of CO₂ and H₂ is of high interest. Therefore, the focus of the present work was to evaluate the performances of two up-flow reactors for CO₂ and H₂ assimilation. Process monitoring showed that the gas-liquid H₂ transfer was highly affected by reactor design. A reactor filled with Raschig rings could lift up gases utilization leading to a CH₄ content of 81% at 6 h gas retention time and 8.8 L/L_R.h gas recirculation rate. In contrast, limited biomethanation was achieved in the absence of Raschig rings highlighting the positive role of packing material to the performance of up-flow-reactors. Additionally, high-throughput 16S rRNA sequencing revealed that the microbial community was ultimately resided by Methanothermobacter methanogens.

作为消除温室气体排放的一种手段，用于生产结构分子和生物燃料的气体发酵近来受到关注。尤其是CO _2的捕获和回收。因此，高度关注CO ₂和H ₂的生物偶联。因此，本工作的重点是评估两个上流反应器对CO ₂和H ₂同化的性能。过程监测表明，气-液H _2的转移受反应器设计的影响很大。装有Raschig环的反应器可以提高气体利用率，导致在6 h气体保留时间和8.8 L / L _R的情况下CH ₄含量为81％.h气体再循环率。相反，在不存在Raschig环的情况下实现了有限的生物甲烷化作用，从而突出了填料对上流反应器性能的积极作用。此外，高通量16S rRNA测序表明，微生物群落最终被产甲烷甲烷杆菌属产甲烷菌占据。