Abstract The in vitro antifungal activity of the liquid fraction obtained from cattle manure pyrolysis was determined. Bio-oils and aqueous phases produced at 400, 500, and 600 °C were tested against two phytopathogenic fungi by incorporating the oil and water phase materials into culture medium, at concentrations of 1, 2, 3, 4, and 5 mg mL−1. The antifungal potentials were determined using colony growth diameters, as compared to controls. The aqueous phase produced at 400 °C and used at a concentration of 4 mg mL−1 provided 100 % inhibition of mycelial growth and was fungicidal against both Lasiodiplodia theobromae and Fusarium solani. The bio-oils produced at all the different temperatures showed fungistatic potential, but there were no statistically significant differences among the products obtained at different temperatures, in terms of their inhibitory activities. Characterization by gas chromatography/mass spectrometry (GC/MS) and ultra-high resolution mass spectrometry (FT-Orbitrap MS) revealed that the materials were composed mainly of classes of oxygenated compounds. The GC/MS analyses showed that phenolic compounds were the main constituents of the bio-oils, with the relative area for these compounds increasing at higher pyrolysis temperatures. The liquid-liquid extraction process did not enable characterization of the aqueous phase by GC/MS, although the FT-MS analyses showed significant differences among the products, with the phase produced at 400 °C mainly presenting classes of aromatic nitrogen compounds. This prospecting study demonstrated the potential of using bio-oils and the aqueous phases from cattle manure pyrolysis as alternative sources of agricultural fungicides.

中文翻译：

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牛粪热解液作为农业杀菌剂替代环保来源的潜力

摘要 测定了从牛粪热解中获得的液体部分的体外抗真菌活性。通过将油相和水相材料以 1、2、3、4 和 5 毫克/毫升的浓度加入培养基中，在 400、500 和 600 °C 下生产的生物油和水相针对两种植物病原真菌进行了测试1. 与对照相比，使用菌落生长直径确定抗真菌潜力。在 400 °C 下产生并以 4 mg mL-1 的浓度使用的水相提供了 100% 的菌丝体生长抑制，并且对 Lasiodiplodia theobromae 和 Fusarium solani 均具有杀菌作用。在所有不同温度下生产的生物油均表现出抑菌潜力，但在不同温度下获得的产品之间没有统计学上的显着差异，就其抑制活性而言。气相色谱/质谱 (GC/MS) 和超高分辨率质谱 (FT-Orbitrap MS) 表征表明，这些材料主要由多种含氧化合物组成。GC/MS 分析表明酚类化合物是生物油的主要成分，这些化合物的相对面积在较高的热解温度下增加。液-液萃取过程无法通过 GC/MS 表征水相，尽管 FT-MS 分析显示产物之间存在显着差异，在 400 °C 下产生的相主要呈现芳香族氮化合物的类别。