Abstract Sporadic and unpredictable extreme hot weather events associated with global warming have been an increasingly serious problem and are difficult to test under natural field conditions. In this study, we used subtropical summer to mimic extreme hot weather under realistic field conditions to test for heat tolerance in the cold-adapted emergent oil crop, Camelina sativa. Utilizing a forward genetic screen, Camelina was screened for heat-adapted genotypes, resulting in the identification of three subtropical summer tolerant (sst) mutants. The sst mutants were late flowering and exhibited altered expression of the key flowering genes FLOWER LOCUS C and FLOWER LOCUS T. With RNA-seq assay, it was found that redox and defense related genes were significantly enriched in the up-regulated genes of the sst mutants. Consistently, reduced hydrogen peroxide production and enhanced resistance to a fungal pathogen were observed. Overall, our results suggested that to breed temperate crops to adapt to the subtropics, flowering time, antioxidant ability, and defense signaling could be the potential targets.

中文翻译：

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改变的氧化还原过程、防御反应和开花时间与亚热带条件下温带亚麻荠的存活有关

摘要 与全球变暖相关的零星和不可预测的极端炎热天气事件已成为一个日益严重的问题，并且难以在自然野外条件下进行测试。在这项研究中，我们使用亚热带夏季来模拟真实田间条件下的极端炎热天气，以测试适应寒冷的挺水油料作物亚麻荠的耐热性。利用正向遗传筛选，筛选了 Camelina 的热适应基因型，从而鉴定了三个亚热带夏季耐受 (sst) 突变体。sst 突变体开花晚，并表现出关键开花基因 FLOWER LOCUS C 和 FLOWER LOCUS T 的表达改变。通过 RNA-seq 分析，发现氧化还原和防御相关基因在 sst 的上调基因中显着富集突变体。一贯地，观察到过氧化氢产生减少和对真菌病原体的抵抗力增强。总的来说，我们的结果表明，培育适应亚热带的温带作物、开花时间、抗氧化能力和防御信号可能是潜在的目标。