The role of parental morphine exposure before gestation on mood disorder in the offspring was well described. Besides, physical activity can improve the symptoms of mood disorders. So, the current study aimed to investigate the role of physical activity on depressive and OCD-like behaviors induced by parental morphine exposure.

40 male and 40 female Wistar rats (60-days old) received morphine for consecutive 10 days and were drug-free for 10 days. They were prepared for mating either with a morphine-abstinent or with a drug-naïve rat. The adult male offspring were divided into two groups as follows: (1) those that were subjected to treadmill exercise for three weeks (3-days each week), and (2) those without exercise. Also, the offspring were subjected to forced swimming and marble-burying tests. The levels of 5-HT₃ receptor (R)_, D1, and D2 dopamine receptor (DR) were evaluated as well as the level of monoamine oxidase-B (MAO-B) in the prefrontal cortex (PFC).

Results showed that exercise improved depressive and OCD-like behaviors in the offspring of morphine-abstinent rats. Western blotting data revealed that the levels of 5-HT₃R, D1DR, D2DR, and MAO-B in the PFC increased in the offspring of morphine-abstinent rats compared to the control. However, it was shown that treadmill exercise decreases the levels of 5-HT₃R, MAO-B, and D2DR.

Morphine exposure, even before conception, could affect the behaviors in the offspring. Besides, the molecular changes were also detected in the brain. We found that mild physical activity might modulate OCD and depressive-like behavior in the offspring of morphine-abstinent rats by decreasing the levels of 5-HT₃R, D2DR, and MAO-B located in the PFC.

父母在妊娠前接触吗啡对后代情绪障碍的作用得到了很好的描述。此外，体育锻炼可以改善情绪障碍的症状。因此，目前的研究旨在调查身体活动对父母吗啡暴露引起的抑郁和强迫症样行为的作用。

40 只雄性和 40 只雌性 Wistar 大鼠（60 天大）连续 10 天接受吗啡，10 天不吸毒。它们准备好与戒除吗啡的大鼠或未使用药物的大鼠交配。成年雄性后代被分为以下两组：（1）那些进行三周（每周3天）跑步机运动的，和（2）那些没有运动的。此外，这些后代还接受了强迫游泳和埋入大理石的测试。评估了 5-HT ₃受体 (R) _、 D1 和 D2 多巴胺受体 (DR) 的水平以及前额叶皮层 (PFC) 中单胺氧化酶-B (MAO-B) 的水平。

结果表明，运动改善了戒除吗啡的大鼠后代的抑郁和强迫症样行为。蛋白质印迹数据显示，与对照相比，吗啡戒断大鼠的后代 PFC中 5-HT ₃ R、D1DR、D2DR 和 MAO-B 的水平增加。然而，结果表明跑步机运动会降低 5-HT ₃ R、MAO-B 和 D2DR 的水平。

吗啡暴露，甚至在受孕之前，就可能影响后代的行为。此外，还在大脑中检测到分子变化。我们发现轻度体力活动可能通过降低PFC中 5-HT ₃ R、D2DR 和 MAO-B的水平来调节戒断吗啡大鼠后代的强迫症和抑郁样行为。