The N⁶-methyladenosine (m6A) derivative has the capacity for ubiquitous epigenetic modification of messenger RNA (mRNA) that regulates gene expression through post-transcriptional mRNA modifications. Findings with mapping of m6A methylomes have indicated there are potential functions of this derivative in different cell types of several species. A profile of m6A methylomes and potential functions in granulosa cells of pigs during antral follicle development, however, has not yet occurred. In the present study, there was profiling of an epitranscriptome-wide map of m6A methylation in granulosa cells of pigs derived from small and large follicles using methylated RNA immunoprecipitation techniques, next-generation sequencing and further annotation of the potential functions of m6A utilizing bioinformatic analyses procedures. The m6A modification is abundant in granulosa cells of pigs, and there are dynamic changes in m6A methylomes during the developmental transition from small (< 3 mm) to large (> 5 mm) sized follicles. In particular, there was a prevalence of 7289 and 6882 m6A in granulosa cells from follicles of two different sizes. There was an increased prevalence of m6A in close proximity to the 5′ or 3′-untranslated coding regions and a shared conserved consensus motif. Results from further analysis indicated there was significant enrichment of differentially expressed m6A methylated genes in several signaling pathways associated with steroidogenesis, granulosa cell proliferation and follicular development. When considered as a whole, these results indicate there are differential m6A modifications in granulosa cells of pigs during follicle development that are potentially associated with steroidogenesis and folliculogenesis.

的Ñ ⁶-甲基腺苷（m6A）衍生物具有通过信使RNA（mRNA）进行无处不在的表观遗传修饰的能力，该信使RNA通过转录后mRNA修饰来调节基因表达。映射m6A甲基化的发现表明，该衍生物在几种物种的不同细胞类型中具有潜在功能。但是，尚未发现猪的卵泡发育过程中m6A甲基化组的概况和猪颗粒细胞的潜在功能。在本研究中，使用甲基化RNA免疫沉淀技术，小分子和大卵泡衍生的猪的颗粒细胞中的m6A甲基化在整个转录组上的图谱分析，下一代测序以及利用生物信息学分析进一步注释了m6A的潜在功能程序。在猪的颗粒细胞中，m6A修饰丰富，并且在从小（<3 mm）到大（> 5 mm）大小的卵泡发育过渡期间，m6A甲基化组存在动态变化。特别地，来自两种不同大小的卵泡的颗粒细胞中普遍存在7289和6882 m6A。紧密靠近5'或3'非翻译编码区和共享的保守共有基序的m6A患病率增加。进一步分析的结果表明，在与类固醇生成，颗粒细胞增殖和卵泡发育相关的几种信号传导途径中，差异表达的m6A甲基化基因大量富集。当整体考虑时，