Skeletal muscle is one of the most important tissues of the human body necessary for sporting activities. The differentiation of muscle-derived satellite cells (MDSCs) plays an important role in the development and regeneration of skeletal muscles. Similarly, the Wnt/β-catenin signalling pathway plays an important role in the process of muscle differentiation. Wnt1-inducible signalling pathway protein-1 (WISP1), a downstream protein of the Wnt/β-catenin signalling pathway and a member of the CCN family that also plays an important role in the differentiation process, and its expression increase during the differentiation of bovine MDSCs. However, its role in MDSC differentiation is poorly understood. Therefore, we investigated the mechanisms regulating this process via Western blot and immunofluorescence staining. Immunoprecipitation and mass spectrometry detected annexin A1 (ANXA1), a protein that interacts with WISP1. To determine whether WISP1 influences TGF-β signalling and differentiation independently of ANXA1, the latter was knocked down, while WISP1 was activated. WISP1 expression increased significantly during bovine MDSC differentiation. However, WISP1 did not affect the TGF-β signalling pathway protein marker when ANXA1 was inhibited. Taken together, WISP1 regulates the TGF-β signalling pathway through ANXA1 recruitment, thereby promoting bovine MDSC differentiation, suggesting the Wnt/β-catenin signalling pathway as another target to promote cell differentiation.

骨骼肌是体育活动所必需的人体最重要的组织之一。肌肉衍生卫星细胞（MDSC）的分化在骨骼肌的发育和再生中起重要作用。同样，Wnt /β-catenin信号通路在肌肉分化过程中也起着重要作用。Wnt1诱导型信号通路蛋白1（WISP1）是Wnt /β-catenin信号通路的下游蛋白，也是CCN家族的成员，在分化过程中也起着重要作用，其表达在Wnt1的分化过程中增加。牛MDSC。但是，人们对它在MDSC分化中的作用了解甚少。因此，我们研究了通过蛋白质印迹和免疫荧光染色调节这一过程的机制。免疫沉淀和质谱检测到膜联蛋白A1（ANXA1），一种与WISP1相互作用的蛋白。为了确定WISP1是否独立于ANXA1影响TGF-β信号传导和分化，将其敲低，同时激活WISP1。在牛MDSC分化过程中，WISP1表达显着增加。但是，当抑制ANXA1时，WISP1不会影响TGF-β信号通路蛋白标记。两者合计，WISP1通过ANXA1募集调节TGF-β信号传导途径，从而促进牛MDSC分化，表明Wnt /β-catenin信号传导途径是促进细胞分化的另一个目标。后者被关闭，而WISP1被激活。在牛MDSC分化过程中，WISP1表达显着增加。但是，当抑制ANXA1时，WISP1不会影响TGF-β信号通路蛋白标记。两者合计，WISP1通过ANXA1募集调节TGF-β信号传导途径，从而促进牛MDSC分化，表明Wnt /β-catenin信号传导途径是促进细胞分化的另一个目标。后者被关闭，而WISP1被激活。在牛MDSC分化过程中，WISP1表达显着增加。但是，当抑制ANXA1时，WISP1不会影响TGF-β信号通路蛋白标记。两者合计，WISP1通过ANXA1募集调节TGF-β信号传导途径，从而促进牛MDSC分化，表明Wnt /β-catenin信号传导途径是促进细胞分化的另一个目标。