The Lactobacillus genus is widely used for fermentation of plant materials and dairy products. These species are typically found in highly specialized environments, with the bee gut serving as one of the niche locations in which Lactobacillus is detected. Lactobacillus species isolated from the bee gut and bee-related habitats were phylogenetically classified into three distinct groups, Lactobacillus kunkeei, Firm-4, and Firm-5. The L. kunkeei group was clearly differentiated from other members of the Lactobacillus buchneri group isolated from non-bee habitats. In comparison with non-bee members of the L. buchneri group, three bee-symbiotic Lactobacillus groups had a small-sized genome with low G + C content and showed a sharp reduction in the number of genes involved in energy production, carbohydrate transport and metabolism, and amino acid transport and metabolism. In addition, all three groups lacked the mutY gene, which encodes A/G-specific adenine glycosylase. The phylogenetic dendrogram based on the presence or absence of 1,199 functional genes indicated that these bee-symbiotic groups experienced convergent evolution. The occurrence of convergent evolution is thought to stem from the three bee-symbiotic groups sharing a similar habitat, i.e., the bee gut. The causative factor underlying genomic reduction was postulated to be mutY, which was absent in all three groups. Here, a novel strain, BHWM-4^T, isolated from the gut of Bombus ignites was studied using polyphasic taxonomy and classified as a new member of the L. kunkeei group. The strain was Gram-positive, facultative anaerobic, and rod-shaped. The 16S ribosomal RNA gene sequence and genome analysis revealed that strain BHWM-4^T was clustered into the L. kunkeei group, forming a compact cluster with L. kunkeei and Lactobacillus apinorum. Biochemical, chemotaxonomic, and genotypic data of strain BHWM-4^T supports the proposal of a novel species, Lactobacillus bombintestini sp. nov., whose type strain is BHWM-4^T (= KACC 19317 = NBRC 113067^T).

中文翻译：

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作为蜜蜂共生体的乳杆菌属物种的比较基因组学和乳杆菌 bombintestini sp 的描述。11 月，从 Bombus ignitus 的肠道中分离出来。

乳杆菌属广泛用于植物材料和乳制品的发酵。这些物种通常存在于高度专业化的环境中，蜜蜂肠道是检测到乳酸杆菌的利基位置之一。从蜜蜂肠道和蜜蜂相关栖息地分离出的乳杆菌属在系统发育上分为三个不同的组：昆氏乳杆菌、Firm-4 和 Firm-5。L. kunkeei组与从非蜜蜂栖息地分离的布氏乳杆菌组的其他成员明显不同。与L. buchneri组的非蜜蜂成员相比，三种蜜蜂共生乳酸杆菌属的基因组较小，G + C 含量较低，参与能量生产、碳水化合物转运和代谢以及氨基酸转运和代谢的基因数量急剧减少。此外，所有三个组都缺少编码 A/G 特异性腺嘌呤糖基化酶的mutY基因。基于 1,199 个功能基因存在与否的系统发育树状图表明，这些蜜蜂共生群体经历了趋同进化。趋同进化的发生被认为源于共享相似栖息地（即蜜蜂肠道）的三个蜜蜂共生群体。基因组减少的致病因素被假定为mutY，这在所有三组中都不存在。在这里，一种新菌株 BHWM-4^{T ，从}Bombus ignites的肠道中分离出来，使用多相分类学进行了研究，并将其归类为L. kunkeei组的新成员。该菌株呈革兰氏阳性、兼性厌氧、杆状。16S核糖体RNA基因序列和基因组分析表明，菌株BHWM-4 ^T被聚类到昆氏乳杆菌群中，与昆氏乳杆菌和Lactobacillus apinorum形成一个紧密的簇。菌株 BHWM-4 ^{T的生化、化学分类学和基因型数据支持新物种}Lactobacillus bombintestini sp . 的提议。nov.，其类型菌株为BHWM-4 ^T（= KACC 19317 = NBRC 113067 ^T）。