Kava is a non-alcoholic beverage prepared by cold water extraction of the ground roots and stumps of Piper methysticum Forst. f. Kava contains flavokawains (FKs) which have been suspected of being potentially cytotoxic. Current HPLC protocols are not adapted to high-throughput quantification before export. The objectives of the present study were (i) to analyse with HPTLC the individual FKs in roots, stump, stems and peelings of four varieties grown in a controlled environment; (ii) to quantify FKs in 1053 commercial samples exported from Vanuatu in 2017-18-19 (370 roots, 381 chips and 302 powders) and (iii) to assess the efficiency of a colorimetric test for routine control. HPTLC plate scanning at 355 nm offered good linearity for three FKs with R² > 0.99 and RSD < 3.0%. High total FKs (> 14 mg/g DW) were found in poor-quality varieties and in peelings unsuitable for consumption. Plant parts known for their good quality, such as roots and peeled stumps of noble varieties, presented low total FKs (< 7 mg/g). Great variation was observed in exported roots (2.53–24.56 mg/g), chips (2.73–18.03 mg/g) and powders (2.92–16.41 mg/g). HPTLC proved reproducible for the high-throughput quantification of FKs in kava. A positive relationship was confirmed between the absorbance of the acetonic extract and the total FKs (R²= 0.5211) (n = 1053). Multivariate analyses revealed that in roots, chips and powders, the three FKs are significantly correlated with high absorbance values. The absorbance of the acetonic extract gives a fair assessment of the FK content in kava products.

卡瓦（Kava）是一种非酒精性饮料，是通过冷水提取胡椒（Piper methysticum Forst）的地根和树桩制成的。F。卡瓦（Kava）含有被认为具有潜在细胞毒性的黄酮类维生素（FK）。当前的HPLC方案不适用于出口前的高通量定量。本研究的目的是（i）用HPTLC分析在受控环境中生长的四个品种的根，树桩，茎和果皮中的单个FK；（ii）量化2017-18-19年从瓦努阿图出口的1053份商业样品中的FK（370根，381片和302粉末），以及（iii）评估比色测试用于常规控制的效率。355 nm的HPTLC板扫描为具有R ^2的三个FK提供了良好的线性> 0.99，RSD <3.0％。在劣质品种和不宜食用的果皮中发现高的总FK（> 14 mg / g DW）。以其优良品质而闻名的植物部位，例如根系和去皮的贵族树桩，其总FK值低（<7 mg / g）。在出口的根（2.53–24.56 mg / g），木片（2.73–18.03 mg / g）和粉末（2.92–16.41 mg / g）中观察到很大的差异。事实证明，HPTLC可对卡瓦中FK进行高通量定量分析。丙酮提取物的吸光度与总FKs之间存在正相关关系（R ² = 0.5211）（n =1053）。多变量分析显示，在根，木片和粉末中，这三种FK与高吸光度值显着相关。丙酮提取物的吸光度可以对卡瓦产品中FK含量进行合理评估。