Hydrophilic interaction liquid chromatography coupled to mass spectrometry was employed for simultaneous determination of vitamin B6 and catechins in dietary supplements. The obtained results clearly shows the potential of the application of alcohol eluent (instead mainly used acetonitrile) as a component of an with zwitterionic stationary phase. The limits of detection on ZIC-HILIC column were 0.01 mg/L for catechins and vitamins B (only for pyridoxal phosphate was 0.10 mg/L). The investigations between green tea extract (GTE) and vitamin B were also evaluated using isobolographic analysis as well as the interaction indexes. Antioxidant activities of single components and their mixtures were determined by DPPH assay. It was found that the mixtures of GTE and vitamin B acted synergistically. In comparison to GTE alone, faster DPPH radical bleaching of the mixtures was observed in the presence of different forms of vitamin B6 (pyridoxine, pyridoxal, or pyridoxal phosphate), particularly for pyridoxal.

中文翻译：

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ZIC-HILIC色谱法同时测定膳食补充剂中的维生素B6和儿茶素及其抗氧化剂相互作用

亲水相互作用液相色谱-质谱联用用于同时测定膳食补充剂中的维生素B6和儿茶素。所获得的结果清楚地表明了将酒精洗脱液（而不是主要使用的乙腈）用作两性离子固定相的组分的潜力。在ZIC-HILIC色谱柱上，儿茶素和维生素B的检出限为0.01 mg / L（仅磷酸吡ido醛的检出限为0.10 mg / L）。绿茶提取物（GTE）和维生素B之间的研究也用等效线描记法分析以及相互作用指数进行了评估。通过DPPH测定法测定单一组分及其混合物的抗氧化活性。发现GTE和维生素B的混合物协同作用。与仅GTE相比，