Introduction

Fibrin scaffolds are often utilized to treat chronic wounds. The monomer fibrinogen used to create such scaffolds is typically derived from adult human or porcine plasma. However, our previous studies have identified extensive differences in fibrin network properties between adults and neonates, including higher fiber alignment in neonatal networks. Wound healing outcomes have been linked to fibrin matrix structure, including fiber alignment, which can affect the binding and migration of cells. We hypothesized that fibrin scaffolds derived from neonatal fibrin would enhance wound healing outcomes compared to adult fibrin scaffolds.

Methods

Fibrin scaffolds were formed from purified adult or neonatal fibrinogen and thrombin then structural analysis was conducted via confocal microscopy. Human neonatal dermal fibroblast attachment, migration, and morphology on fibrin scaffolds were assessed. A murine full thickness injury model was used to compare healing in vivo in the presence of neonatal fibrin, adult fibrin, or saline.

Results

Distinct fibrin architectures were observed between adult and neonatal scaffolds. Significantly higher fibroblast attachment and migration was observed on neonatal scaffolds compared to adults. Cell morphology on neonatal scaffolds exhibited higher spreading compared to adult scaffolds. In vivo significantly smaller wound areas and greater epidermal thickness were observed when wounds were treated with neonatal fibrin compared to adult fibrin or a saline control.

Conclusions

Distinctions in neonatal and adult fibrin scaffold properties influence cellular behavior and wound healing. These studies indicate that fibrin scaffolds sourced from neonatal plasma could improve healing outcomes compared to scaffolds sourced from adult plasma.

中文翻译：

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与成人纤维蛋白支架相比，新生儿纤维蛋白支架可促进体内细胞粘附、迁移和伤口愈合增强

 介绍

纤维蛋白支架通常用于治疗慢性伤口。用于创建此类支架的单体纤维蛋白原通常源自成年人或猪血浆。然而，我们之前的研究已经发现成人和新生儿之间的纤维蛋白网络特性存在巨大差异，包括新生儿网络中纤维蛋白排列程度较高。伤口愈合结果与纤维蛋白基质结构有关，包括纤维排列，它可以影响细胞的结合和迁移。我们假设，与成人纤维蛋白支架相比，源自新生儿纤维蛋白的纤维蛋白支架能够增强伤口愈合效果。

 方法

纤维蛋白支架由纯化的成人或新生儿纤维蛋白原和凝血酶形成，然后通过共聚焦显微镜进行结构分析。评估了人新生儿真皮成纤维细胞在纤维蛋白支架上的附着、迁移和形态。使用小鼠全层损伤模型来比较新生儿纤维蛋白、成人纤维蛋白或盐水存在下的体内愈合情况。

 结果

在成人和新生儿支架之间观察到不同的纤维蛋白结构。与成人相比，在新生儿支架上观察到显着更高的成纤维细胞附着和迁移。与成人支架相比，新生儿支架上的细胞形态表现出更高的扩散性。在体内，与成人纤维蛋白或盐水对照相比，用新生儿纤维蛋白治疗伤口时观察到明显更小的伤口面积和更大的表皮厚度。

 结论

新生儿和成人纤维蛋白支架特性的差异影响细胞行为和伤口愈合。这些研究表明，与源自成人血浆的支架相比，源自新生儿血浆的纤维蛋白支架可以改善愈合结果。